2-巯基乙醇
货号:M3148-25ML,M3148-100ML,M3148-250ML,M3148-500ML
规格:25 mL,100 mL,250 mL,500 mL
价格:230,693,983,1795
产品类型:基础细胞培养和血清
品牌:Sigma
还原剂β-巯基乙醇或2-巯基乙醇已用于:•作为Roswell Park Memorial Institute (RPMI)-1640培养基的一种补充剂用于DT40细胞(鸡B细胞系)的培养•裂解NuLi-1(Normal Lung, University of Iowa-1)细胞•小鼠胚胎干细胞的胰蛋白酶消化BME 用于在聚丙烯酰胺凝胶电泳前还原蛋白质二硫键,通常以 5% 的浓度包含在用于 SDS-PAGE 的样本缓冲液中。裂解分子间(亚基之间)的二硫键,从而使蛋白质亚基在 SDS-PAGE 上各自分开。裂解分子内(亚基内)的二硫键从而使亚基完全变性,以便每个肽按照其链长迁移而不受二级结构的影响。 |
性质
Related Categories | Antioxidants and Reducing Agents for Protein Stabilization,Core Bioreagents,General Reagents,Life Science Reagents for Northern and Southern Blotting,Life Science Reagents for Nucleic Acid Purification, 更多... |
Quality Level | 200 |
grade | for electrophoresis |
| for molecular biology |
vapor density | 2.69 (vs air) |
vapor pressure | 1 mmHg ( 20 °C) |
product line | BioReagent |
assay | 99% (GC/titration) |
form | liquid |
expl. lim. | 18% |
concentration | 14.3M (pure liquid) |
application(s) | cell culture | mammalian: suitable |
refractive index | n20/D1.500 (lit.) |
pH | 4.5-6 (20°C, 500g/L) |
bp | 157°C (lit.) |
solubility | H2O: soluble 1mL/mL |
density | 1.114g/mLat 25°C (lit.) |
foreign activity | DNase, RNase, protease, none detected |
storage temp. | room temp |
SMILES string | OCCS |
InChI | 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2 |
InChI key | DGVVWUTYPXICAM-UHFFFAOYSA-N |
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