DH5α Competent Cells for Subcloning/DH5α 感受态细胞用于亚克隆
货号：EC0111
规格：4 x 500 µL
价格：563
产品类型：感受态细胞
品牌：Thermo Fisher
| Thermo Scientific DH5α Competent Cells for Subcloning are chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal.• Transformation efficiency: >1x106cfu/µg pUC19 DNA• Suitable for routine cloning applications• Convenient economical packaging• Contain genetic markers that allow for blue/white colony screeningApplicationsDH5α Competent Cells for Subcloning are suitable for the standard DNA cloning applications:• DNA cloning following PCR or restriction digestion reactions• Subcloning (transferring DNA from a parent vector to a destination vector)• Plasmid isolationGenotypeF– φ80lacZΔ M15 Δ (lacZYA-argF)U169 recA1 endA1 hsdR17(rK– mK+)phoA supE44λ-thi–1 gyrA96 relA1 |
优点： |
| - Transformation efficiency: >1x106cfu/µg pUC19 DNA- Suitable for routine cloning applications- Convenient economical packaging- Contain genetic markers that allow for blue/white colony screening |
组成成分： |
| ▪DH5α Competent Cells for Subcloning ▪pUC19 DNA (100 pg/µL) |
相关产品： |
| ▪DH5α Competent Cells for Subcloning/DH5α 感受态细胞用于亚克隆（货号#EC0111)▪DH5α Competent Cells/DH5α 感受态细胞（货号#EC0112）▪DH10B Competent Cells/DH10B 感受态细胞（货号#EC0113）▪BL21(DE3) Competent Cells/BL21(DE3)感受态细胞（货号#EC0114） |
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