| Thermo Scientific BveI (BspMI) restriction enzyme recognizes ACCTGC(4/8)^ sites and cuts best at 37°C in O (+oligo) buffer (isoschizomers: Acc36I, BfuAI, BspMI). SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as aFastDigest enzymefor rapid DNA digestion.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Note:s At least two copies of BveI recognition site are required for efficient cleavage. Inclusion of 0.5µM oligonucleotide with the BveI recognition sequence in the reaction mixture significantly improves cleavage of plasmid DNAs, especially of those with a single BveI site. Still, complete cleavage of some substrates with BveI is difficult to achieve. BveI concentration is determined by the maximum cleavage level achieved when no change in the fragmentation pattern is observed with further enzyme increase. Low salt, high glycerol (>5%) concentrations, pH>8.0, or a large excess of enzyme may result in star activity. BveI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis. For methylation sensitivity, refer to product specifications.Thermo Scientific BveI（BspMI）限制酶识别ACCTGC（4/8）^位点，并在37°C的O种（+ oligo）缓冲液（同分异构体：Acc36I，BfuAI，BspMI）中切割效果最佳。有关此酶及其他限制酶的酶活性，双重消化条件和热灭活条件，请参见限制酶的反应条件表格。注意：也可以作为FastDigest酶用于快速DNA消化。Thermo Scientific常规限制性内切核酸酶是大量高质量的限制性内切酶，经过优化可在五种缓冲液系统的一种缓冲液中工作。 此外，通用的Tango缓冲液为双消化提供了便利。 在推荐的缓冲液和反应条件下，所有酶均具有100％的活性。 为确保性能稳定，Thermo Scientific限制性内切酶反应缓冲液包含预混合的BSA，可增强许多酶的稳定性并结合DNA制备物中可能存在的污染物。注意：有效裂解需要至少两个BveI识别位点。 在反应混合物中加入具有BveI识别序列的0.5 µM寡核苷酸可显着改善质粒DNA的裂解，尤其是具有单个BveI位点的质粒DNA的裂解。 仍然难以用BveI完全裂解某些底物。BveI浓度由在进一步酶增加时未观察到片段化模式变化的最大裂解水平决定。 低盐，高甘油（> 5％）浓度，pH> 8.0或酶大量过量可能导致星号活性。 BveI可保持与切割的DNA缔合。 这可能会在电泳过程中引起DNA条带移动。 为了避免出现非典型的DNA条带图谱，请在电泳前使用6X DNA上样染料和SDS溶液进行样品制备或在SDS存在的情况下加热消化的DNA。 有关甲基化敏感性，请参阅产品说明。 |
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