SIGLEC H Monoclonal Antibody (eBio440c), FITC

2024-10-18

SIGLEC H Monoclonal Antibody (eBio440c), FITC

货号:11-0333-82

规格:100μg

价格:1707

产品类型:流式抗体

品牌:eBioscience

物种:小鼠

宿主:大鼠

抗体亚型:IgG2b, kappa

克隆号:eBio440c

荧光染料:FITC

类型:单抗同型对照:Rat IgG2b kappa Isotype Control (eB149/10H5), FITC
浓度:0.5 mg/mL用法:0.25 μg/test
产品详细信息Description: The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction.The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG).It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.Applications Reported: This eBio440c antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio440c antibody has been tested by flow cytometric analysis of SJL mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction. The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG). It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.
数据
SIGLEC H Antibody (11-0333-82) in FlowStaining of SJL splenocytes with Anti-Human/Mouse CD45R (B220) PE (Product # 12-0452-82) (left), Anti-Mouse CD317 (BST2, PDCA-1) APC (right) and 0.125 µg of Anti-Mouse Siglec H FITC. Cells in the large forward scatter population were used for analysis.
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参考文献:

1.The Journal of experimental medicineDC subset-specific induction of T cell responses upon antigen uptake via Fcγ receptors in vivo."11-0333 was used in Flow cytometry/Cell sorting to suggest that targeting antigens to FcγRs on DCs may be a promising approach to raise immunogenic or tolerogenic T cell responses to antigens of choice."AuthorsLehmann CHK,Baranska A,Heidkamp GF,Heger L,Neubert K,Lühr JJ,Hoffmann A,Reimer KC,Brückner C,Beck S,Seeling M,Kießling M,Soulat D,Krug AB,Ravetch JV,Leusen JHW,Nimmerjahn F,Dudziak D2.The Journal of allergy and clinical immunologyInnate lymphoid cells contribute to allergic airway disease exacerbation by obesity."11-0333 was used in Flow cytometry/Cell sorting to indicate that high-fat diet-induced obesity might exacerbate allergic airway inflammation through mechanisms involving ILC2s and ILC3s."AuthorsEveraere L,Ait-Yahia S,Molendi-Coste O,Vorng H,Quemener S,LeVu P,Fleury S,Bouchaert E,Fan Y,Duez C,de Nadai P,Staels B,Dombrowicz D,Tsicopoulos A3.Journal of immunology (Baltimore, Md. : 1950)Canonical and noncanonical Wnt proteins program dendritic cell responses for tolerance."11-0333 was used in Flow cytometry/Cell sorting to investigate the role of canonical and noncanonical Wnts in immune tolerance, showing that they program dendritic cell responses for tolerance."AuthorsOderup C,LaJevic M,Butcher EC4.Mucosal immunologyCD8α⁺β⁻ and CD8α⁺β⁺ plasmacytoid dendritic cells induce Foxp3⁺ regulatory T cells and prevent the induction of airway hyper-reactivity."11-0333 was used in Flow cytometry/Cell sorting to reveal three functional and phenotypically distinct populations of mice plasmacytoid dendritic cells."AuthorsLombardi V,Speak AO,Kerzerho J,Szely N,Akbari O5.ImmunityPlasmacytoid dendritic cells transport peripheral antigens to the thymus to promote central tolerance."11-0333 was used in Flow cytometry/Cell sorting to explore the mechanisms underlying the contribution to immune tolerance conducted by peripheral dendritic cells."AuthorsHadeiba H,Lahl K,Edalati A,Oderup C,Habtezion A,Pachynski R,Nguyen L,Ghodsi A,Adler S,Butcher EC
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