DreamTaq™ Hot Start PCR Master Mix/热启动PCR混合液
货号：K9011,K9012
规格：200 rxns,1000 rxns
价格：881,3981
产品类型：PCR 及DNA聚合酶
品牌：Thermo Fisher
| Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq™ Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. A version of this master mix is also available with pre-added density reagent and electrophoresis tracking dyes.DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases. DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases.DreamTaq Hot Start PCR Master Mix is formulated to enhance productivity:• Better reaction outcomes--Higher yield of target amplicons from low template amounts--Increased reaction specificity due to reliable hot-start technology--Wider range of amplicon lengths - routine amplification of genomic DNA fragments up to 6kb• Enhanced convenience--Room temperature reaction set-up--Minimized optimization of Mg2+ concentration and of primer annealing temperatures due to optimized reaction buffer--Simplified workflow and minimized pipetting steps with 2X master mix format |
组成成分： |
| ▪ 4 x 1.25 mL DreamTaq Hot Start PCR Master Mix (2X)▪ 4 x 1.25 mL Nuclease-free waterStore at -5 to -30°C. |
相关产品： |
| ▪DNA 片段标准品▪DNA ladder▪DNA 聚合酶▪PCR▪DNA纯化▪RNA纯化 |
技术参数 产品应用 • Routine PCR
• Colony PCR
• Genotyping
• RT-PCR
• Generation of PCR products for TA cloning
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