CD69 Monoclonal Antibody (FN50), eBioscience™

admin 2024-10-18 16

CD69 Monoclonal Antibody (FN50), eBioscience™

货号:14-0699-82,14-0699-37,14-0699-95

规格:100μg,2mg,10mg

价格:1136,11562,28906

产品类型:流式抗体

品牌:Thermo Fisher

抗原:CD69

物种:人

宿主:小鼠

抗体亚型:IgG1, kappa

克隆号:FN50

荧光染料:其它

抗体类型:流式抗体同型对照:IgG1, kappa
浓度: 0.5 mg/mL用法:0.5 µg/test (Flow)
产品详细信息Description:The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.Applications Reported: The FN50 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining.Applications Tested: The FN50 antibody has been tested by flow cytometric analysis of resting and 6-hour TPA-activated human normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据

CD69 Antibody (14-0699-82)eLife 2015 -Figure 1--figure supplement 4. Tat-induced transcriptome changes are also observed at the protein level. Flow cytometry analysis of Jurkat-GFP and -Tat cell lines induced with doxycycline for 24 hr and stained with CD4-APC and CD69-PE antibodies or unstained (negative control) to monitor levels of CD4 and CD69 proteins expressed at the cell surface. Note the increase in the CD69+ population in the presence of Tat. GFP, green fluorescent protein.
推荐产品:
二抗荧光二抗免疫组化一抗标签抗体
参考文献:
1.Scientific reportsCombinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation."Published figure using CD69 monoclonal antibody (Product # 14-0699-82) in Flow Cytometry"2.Nature communicationsRSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection."Published figure using CD69 monoclonal antibody (Product # 14-0699-82) in Flow Cytometry"3.The Journal of clinical investigationMucosal-associated invariant T cell alterations in obese and type 2 diabetic patients."14-0699 was used in Flow cytometry/Cell sorting to analyse MAIT cells in the blood and adipose tissues of patients with type 2 diabetes and/or severe obesity."

技术参数
© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:https://biorod.cn/thread-27215.htm
产品询价需求提交
最新回复 (0)
返回
发布信息
扫码添加客服微信,咨询产品报价