Maxima H Minus Double-Stranded cDNA Synthesis Kit/双链cDNA合成试剂盒
货号：K2561,K2562,K2563
规格：10 reactions,50 reactions,200 reactions
价格：5338,19145,41342
产品类型：核酸合成
品牌：Thermo Fisher
| Thermo Scientific Maxima H Minus 双链 cDNA 合成试剂盒是一套可从总 RNA 或 mRNA中高效合成双链 cDNA 的完整系统。第一链和第二链 cDNA 合成反应在同一管内进行，无需中间有机萃取或乙醇沉淀步骤。这种便利的单管形式可加速合成程序并较大限度提高 cDNA 回收率。该试剂盒包含预混组分，减少了完成该程序所需的移液步骤。Maxima H Minus 双链 cDNA 合成试剂盒的特点包括：•高效合成全长双链 cDNA•快速—在不到两小时内完成程序•便利—预混合组分•完整性—包括所有引物、对照品和残留 RNA 去除试剂应用•适用于克隆的全长双链平末端 cDNA 合成•双链 cDNA 文库构建 |
| 数据 |
| 20 µg of total Jurkat RNA, was used as a template for oligo-dT primed first strand and then second strand cDNA synthesis using Maxima H Minus Double-stranded cDNA synthesis kit and the kits from other vendors according to manufacturers instructions. Yields of purified reaction products were estimated using NanoDrop® spectrophotometer (calculated based on assumption that mRNA accounts for 5% of total RNA) (1a). One half of the each reaction product analyzed on agarose gel (1b).<br/>M – ZipRuler Express DNA Ladder Mix<br/>ss – single-stranded cDNA<br/>ds – double-stranded cDNA |
相关产品： |
| ▪DNA 片段标准品▪DNA ladder▪DNA 聚合酶▪PCR▪DNA纯化▪RNA纯化 |
技术参数 产品应用 • Full-length double-stranded blunt-end cDNA synthesis for cloning
• Double-stranded cDNA library construction
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