First Strand cDNA Synthesis Kit/第一条cDNA合成试剂盒
货号：K1612
规格：100 reactions
价格：0
产品类型：核酸合成
品牌：Thermo Fisher
| Thermo Scientific First Strand cDNA Synthesis kit utilizes the recombinant M-MuLV Reverse Transcriptase which exhibits lower RNase H activity than AMV reverse transcriptase. Due to this feature, full-length cDNA can be synthesized from RNA templates up to 9 kb. The recombinant RiboLock RNase Inhibitor, supplied with the kit effectively protects RNA template from degradation.The first strand of cDNA can be directly used as a template in PCR or in second strand cDNA synthesis.The kit is supplied with both oligo(dT)18and random hexamer primers. The oligo(dT)18anneals selectively on the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis using RNA without poly(A) tail e.g. micro RNAs. Gene-specific primers may also be used with the kits. |
优点： |
| - Efficient synthesis of first strand cDNA up to 9 kb- Reaction temperature 37°C- Supplied with the recombinantRiboLock RNase Inhibitor- Complete—oligo(dT)18andrandom hexamerprimers included with the kit |
数据： |
图1.Full-length cDNA synthesis using the First Strand cDNA Synthesis Kit Total RNA isolated from the patient blood infected with hepatitis C virus was used as a template in a reverse transcription reaction using First Strand cDNA Synthesis Kit. An oligonucleotide specific to the 3’-end of the viral RNA was used as a primer. The synthesized 9.4kb cDNA was used as a template in subsequent PCR reactions with Taq DNA Polymerase. The ability to amplify the terminal 212bp sequence indicates that the entire 9.4kb viral RNA sequence was reverse-transcribed. |
组成成分： |
| ▪M-MuLV Reverse Transcriptase▪ RiboLock RNase Inhibitor▪ 5X Reaction Buffer▪ dNTP Mix, 10 mM each▪ Oligo(dT)18Primer▪ Random Hexamer Primer▪ Control GAPDH RNA▪ Forward GAPDH Primer, 10 µM (5'-CAAGGTCATCCATGACAACTTTG-3')▪ Reverse GAPDH Primer, 10 µM (5'-GTCCACCACCCTGTTGCTGTAG-3')▪ Water, nuclease freeStore at -20°C. |
相关产品： |
| ▪DNA 片段标准品▪DNA ladder▪DNA 聚合酶▪PCR▪DNA纯化▪RNA纯化 |
技术参数 产品应用 • First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Construction of cDNA libraries
• Generation of probes for hybridization
• aRNA synthesis
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