CD3 Monoclonal Antibody (17A2), Functional Grade, eBioscience™/CD3 单抗(17A2),功能抗体, eBioscience™

货号：16-0032-81,16-0032-82,16-0032-85,16-0032-86,16-0032-38,16-0032-025,16-0032-050

规格：50µg,100µg,500µg,1 mg,5mg,25mg,50mg

价格：934,1365,2628,4803,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: This 17A2 antibody has been reported for use in flow cytometric analysis and functional activity (in vitro simulation).Applications Tested: The 17A2 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components havelong cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD3 Antibody (16-0032-81) in FlowStaining of mouse thymocytes with Anti-Mouse CD3 Purified followed by Anti-Rat IgG FITC.Appropriate isotype controls were used (open histogram).Total viable cells were used for analysis.

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参考文献：

1.Journal for immunotherapy of cancerMDM2 inhibitor APG-115 synergizes with PD-1 blockade through enhancing antitumor immunity in the tumor microenvironment."Published figure using CD3 monoclonal antibody (Product # 16-0032-82) in Flow Cytometry"2.mSphereMicrobiota Reconstitution Does Not Cause Bone Loss in Germ-Free Mice."Published figure using CD3 monoclonal antibody (Product # 16-0032-82) in Flow Cytometry"3.International journal of molecular medicineDownregulation of Blimp1 inhibits the maturation of bone marrow-derived dendritic cells."16-0032 was used in Flow cytometry/Cell sorting to demonstrate that transduction with lenti-shRNA-Blimp1 (lentiviral-mediated B-lymphocyte-induced maturation protein-1 shRNA) modulated the maturation of DCs via MHC-II molecule suppression and inhibited alloreactive T cell activation."AuthorsXiao J,Zhang J,Li X,Dai X,Wang J,He Y,Wei L,Shi J,Gong N4.Journal of immunology (Baltimore, Md. : 1950)Characterization of conventional and atypical receptors for the chemokine CCL2 on mouse leukocytes."16-0032 was used in Flow cytometry/Cell sorting to define the expression profile, scavenging activity, and ligand specificity of CCL2 receptors on mouse leukocytes."AuthorsFord LB,Cerovic V,Milling SW,Graham GJ,Hansell CA,Nibbs RJ5.BoneOsteoclast-induced Foxp3+ CD8 T-cells limit bone loss in mice."16-0032 was used in Flow cytometry/Cell sorting to provide in vivo evidence that osteoclast-induced Treg cells have anti-resorptive activity and repress the immune system."AuthorsBuchwald ZS,Kiesel JR,Yang C,DiPaolo R,Novack DV,Aurora R

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