CD40 Monoclonal Antibody (5C3), Functional Grade, eBioscience™/CD40 单抗 (5C3), 功能抗体, eBioscience™

货号：16-0409-81,16-0409-85,16-0409-38,16-0409-025,16-0409-050

规格：50 µg,500 µg,5mg,25mg,50 mg

价格：1283,3847,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD40

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号： 5C3

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1 µg/test（Flow）

产品详细信息Description: The 5C3 monoclonal antibody reacts with human CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development and maturation by inducing Ig isotype-switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. 5C3 is reported to be used for activation of B cells in in vitro functional assays.Applications Reported: The 5C3 antibody has been reported for use in flow cytometric analysis. 5C3 has also been reported in in vitro functional studies.Applications Tested: The 5C3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD40 Antibody (16-0409-81) in FlowStaining of normal human peripheral blood cells with Anti-Human CD40 FITC (left) or PE (right).Appropriate isotype controls were used (open histogram).Cells in the lymphocyte population were used for analysis.

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参考文献：

流式1.BloodSevere loss of invariant NKT cells exhibiting anti-HTLV-1 activity in patients with HTLV-1-associated disorders."16-0409 was used in Flow cytometry/Cell sorting to assess the contribution of iNKT cells to the immune defence against human T-lymphotropic virus type 1 and associated disorders."AuthorsAzakami K,Sato T,Araya N,Utsunomiya A,Kubota R,Suzuki K,Hasegawa D,Izumi T,Fujita H,Aratani S,Fujii R,Yagishita N,Kamijuku H,Kanekura T,Seino K,Nishioka K,Nakajima T,Yamano Y2.Journal of immunology (Baltimore, Md. : 1950)Endothelial microparticles interact with and support the proliferation of T cells."16-0409 was used in Flow cytometry/Cell sorting to explore the effect of endothelial cell microparticles in T cell activation and antigen presentation."AuthorsWheway J,Latham SL,Combes V,Grau GE功能检测3.Scientific reportsTranscriptional and functional characterization of CD137L-dendritic cells identifies a novel dendritic cell phenotype."16-0409 was used in Functional assays to characterise the gene expression profile of CD137 ligand (CD137L)-dendritic cells (DCs), and identify significant similarities of CD137L-DCs with in vivo inflammatory monocyte-derived DCs and macrophages."AuthorsHarfuddin Z,Dharmadhikari B,Wong SC,Duan K,Poidinger M,Kwajah S,Schwarz H4.Arthritis and rheumatology (Hoboken, N.J.)A CD8 T cell/indoleamine 2,3-dioxygenase axis is required for mesenchymal stem cell suppression of human systemic lupus erythematosus."16-0409 was used in Functional assays to highlight a previously unrecognised CD8+ T cell/IFNγ/IDO axis that mediates the therapeutic effects of allogeneic MSCs in lupus patients."AuthorsWang D,Feng X,Lu L,Konkel JE,Zhang H,Chen Z,Li X,Gao X,Lu L,Shi S,Chen W,Sun L5.European journal of immunologyCD46-induced human Treg enhance B-cell responses."16-0409 was used in Functional assays to analyse the effect of cTreg on B-cell functions in a co-culture system."AuthorsFuchs A,Atkinson JP,Fremeaux-Bacchi V,Kemper C

技术参数

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