CD4 Monoclonal Antibody (RPA-T4), Functional Grade, eBioscience™/CD4 单抗(RPA-T4), 功能抗体, eBioscience™

货号：16-0049-85,16-0049-38,16-0049-025,16-0049-050

规格：500 µg,5 mg,25 mg ,50 mg

价格：3779,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD4

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：RPA-T4

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	Assay-Dependent（Flow）；Assay-Dependent（Neu）；Assay-Dependent（FN）

产品详细信息Description: The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.Applications Reported: The RPA-T4 antibody has been reported for use in flow cytometric analysis. RPA-T4 has also been reported for use in blocking of CD4 activity in in vitro functional assays.Applications Tested: The RPA-T4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD4 Antibody (16-0049-85) in FlowStaining of human peripheral blood lymphocytes with Anti-Human CD4 Functional Grade Purified followed by Anti-Mouse IgG FITC (Product # 11-4011-85).

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参考文献：

流式1.Molecular medicine reportsMicroRNA‑155 inhibits the proliferation of CD8+ Tcells via upregulating regulatory T cells in vitiligo."Published figure using CD4 monoclonal antibody (Product # 16-0049-85) in Flow Cytometry"2.CellRAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses."Published figure using CD4 monoclonal antibody (Product # 16-0049-85) in Flow Cytometry"3. CellNon-neutralizing Antibodies Alter the Course of HIV-1 Infection InVivo."Published figure using CD4 monoclonal antibody (Product # 16-0049-85) in Flow Cytometry"中和4.RetrovirologyGelsolin activity controls efficient early HIV-1 infection."16-0049 was used in Neutralization experiments to propose that gelsolin can limit HIV-1 infection at a pre-fusion step."AuthorsGarcía-Expósito L,Ziglio S,Barroso-González J,de Armas-Rillo L,Valera MS,Zipeto D,Machado JD,Valenzuela-Fernández AIF5.Proceedings of the National Academy of Sciences of the United States of AmericaRemarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions."Published figure using CD4 monoclonal antibody (Product # 16-0049-85) in Flow Cytometry"

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