SuperScript One-Cycle cDNA Kit for use with Affymetrix One-Cycle Assays

货号：A10752030

规格：30 reactions

价格：40594

产品类型：核酸标记

品牌：Thermo Fisher

The SuperScript One-Cycle cDNA Kit for use with Affymetrix One-Cycle Assays conveniently contains all of the reagents required for double-stranded cDNA synthesis within the Affymetrix GeneChip workflow. The kit includes Invitrogen’s proprietary RNase H reduced activity SuperScript II Reverse Transcriptase and a qualified oligo (dt)-containing primer to perform reactions standard to Affymetrix’ protocol. Designed to consistently produce higher yields of full-length cDNA for Affymetrix One-Cycle assays using either total RNA or poly A+ selected RNA (mRNA).

技术参数

组成成分 50 µM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10 mM dNTP, SuperScript™ II (200u⁄ul), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, RNase H, T4 DNA Polymerase, 0.5M EDTA, RNase-free WaterAll components are guaranteed stable for 6 months when properly stored.

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