Qubit dsDNA HS Assay Kit

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Qubit dsDNA HS Assay Kit

货号:Q32851,Q32854

规格:100 assays,500 assays

价格:1377,4135

产品类型:核酸标记

品牌:Thermo Fisher

The Qubit dsDNA HS (High Sensitivity) Assay Kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.
数据

Increase in accuracy and precision using the Qubit®Accuracy and precision of the Qubit® Quantitation Platform. Ten replicates of lambda DNA at concentrations from 0.01 to 10 ng/µl were assayed using the Qubit® DNA HS Assay on the Qubit® 2.0 Fluorometer according to the standard kit protocol. The same concentrations of DNA were measured in 10 replicates using a NanoDrop® ND-1000 Spectrophotometer, and results were compared for both accuracy and precision. Each bar represents the average of 10 replicates. Error bars represent the standard deviations of the 10 replicates. The concentrations indicated are the concentrations of DNA in the starting samples, before dilution in the Qubit® assay tubes.

Performance of the Qubit dsDNA HS AssayPerformance of the Qubit dsDNA HS Assay. The Qubit dsDNA HS Assay has a linear detection range of 0.2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA.

技术参数
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