CD278 (ICOS) Monoclonal Antibody (C398.4A), Functional Grade, eBioscience™/CD278 (ICOS) 单克隆 (C398.4A), 功能抗体, eBioscience™

货号：16-9949-82,16-9949-38,16-9949-025,16-9949-050

规格：100 µg,5 mg,25 mg,50 mg

价格：3274,999999999,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD278

物种：恒河猴/小鼠/大鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：C398.4A

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The C398.4A monoclonal antibody reacts with ICOS (Inducible Costimulatory molecule), also known as H4/CRP-1/AILIM, and cross-reacts with mouse and rat ICOS. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. A dimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1, is thought to play a protective role in inflammatory autoimmune diseases and be involved in the development of Th2 cells. C398.4A is reported to display strong costimulation of proliferation of T cell clones, intermediate costimulation on activated T cells, and weak costimulation on fresh resting T cells. It is a good costimulator of IL-10 production especially from pre-activated cells that express high level of H4/ICOS. The epitope recognized by C398.4A is different from that bound by the ICOSL (i.e., C398.4A does not block binding of ICOSL).Applications Reported: The C398.4A antibody has been reported for use in flow cytometric analysis. This has also been reported for in vitro functional assays.Applications Tested: This C398.4A antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T-cells. It is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. It enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells.仅用于科研。不用于诊断过程。

数据

CD278 (ICOS) Antibody (16-9949-82) in FlowStaining of 2-day Con A-stimulated mouse splenocytes with 0.25 µg Anti-Mouse/Rat CD278 (ICOS) Purified (filled histogram) or Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) followed by Anti-Armenian Hamster IgG FITC (Product # 11-4111). Total viable cells were used for analysis.

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参考文献：

流式1.Frontiers in immunologyThe Kinase Complex mTOR Complex 2 Promotes the Follicular Migration and Functional Maturation of Differentiated Follicular Helper CD4+T Cells During Viral Infection."Published figure using CD278 (ICOS) monoclonal antibody (Product # 16-9949-82) in Flow Cytometry"2.Nature communicationsDifferential cell-intrinsic regulations of germinal center B and T cells by miR-146a and miR-146b."Published figure using CD278 (ICOS) monoclonal antibody (Product # 16-9949-82) in Flow Cytometry"3.Frontiers in immunologyMicroRNA-155 Protects Group 2 Innate Lymphoid Cells From Apoptosis to Promote Type-2 Immunity."Published figure using CD278 (ICOS) monoclonal antibody (Product # 16-9949-82) in Flow Cytometry"阻断4.ImmunityICOS coreceptor signaling inactivates the transcription factor FOXO1 to promote Tfh cell differentiation."16994982 was used in blocking or activating experiment to explore the relationship between ICOS signaling and FOXO1 in T follicular helper cells"AuthorsStone EL,Pepper M,Katayama CD,Kerdiles YM,Lai CY,Emslie E,Lin YC,Yang E,Goldrath AW,Li MO,Cantrell DA,Hedrick SM

技术参数

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