CD27 Monoclonal Antibody (LG.7F9), Functional Grade, eBioscience™/CD27 单克隆 (LG.7F9), 功能抗体, eBioscience™

货号：16-0271-85,16-0271-025,16-0271-050

规格：500 µg,25 mg,50 mg

价格：3434,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD27

物种：人/小鼠/大鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：LG.7F9

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	IgG3, kappa
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.7F9 cross-reacts with human and rat CD27.Applications Reported: The LG.7F9 antibody has been reported for use in flow cytometric analysis. LG.7F9 has also been reported in blocking of ligand binding.Applications Tested: The LG.7F9 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.仅用于科研。不用于诊断过程。

数据

CD27 Antibody (16-0271-85) in FlowStaining of BALB/c splenocytes with 0.25 µg Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) or 0.25 µg Anti-CD27 Functional Grade Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC (Product # 11-4111).Total viable cells were analyzed.

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参考文献：

流式1.Nature communicationsNK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation."Published figure using CD27 monoclonal antibody (Product # 16-0271-85) in Flow Cytometry"2.eLifeTissue-resident natural killer (NK) cells are cell lineages distinct from thymic and conventional splenic NK cells."16-0271 was used in Flow cytometry/Cell sorting to investigate whether tissue-resident and cells resembling conventional splenic natural killer cells natural killer cells are derived from the same lineage, showing that there are at least four distinct lineages."AuthorsSojka DK,Plougastel-Douglas B,Yang L,Pak-Wittel MA,Artyomov MN,Ivanova Y,Zhong C,Chase JM,Rothman PB,Yu J,Riley JK,Zhu J,Tian Z,Yokoyama WM3.Journal of immunology (Baltimore, Md. : 1950)CD8 T cells recruited early in mouse polyomavirus infection undergo exhaustion."16-0271 was used in Flow cytometry/Cell sorting to characterise virus-specific CD8 T cells recruited during persistent infection."AuthorsWilson JJ,Pack CD,Lin E,Frost EL,Albrecht JA,Hadley A,Hofstetter AR,Tevethia SS,Schell TD,Lukacher AE4.The Journal of experimental medicineT cell-independent development and induction of somatic hypermutation in human IgM+ IgD+ CD27+ B cells."16-0271 was used in Flow cytometry/Cell sorting to demonstrate that development and induction of SHMs of human IgM+IgD+CD27+ B cells can occur in a T cell-independent manner."AuthorsScheeren FA,Nagasawa M,Weijer K,Cupedo T,Kirberg J,Legrand N,Spits H功能检测5.Journal of immunology (Baltimore, Md. : 1950) Characterization of murine CD70, the ligand of the TNF receptor family member CD27. AuthorsTesselaar K,Gravestein LA,van Schijndel GM,Borst J,van Lier RA

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