Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

货号： 12-4714-41, 12-4714-42

规格：25 µg,100 µg

价格：635,1277

产品类型：小鼠同型对照

品牌：eBioscience

物种：小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：P3.6.2.8.1

荧光染料：PE

类型:	同型对照	同型对照：
浓度：	5 µL/test	用法：	Assay-Dependent(Ctrl)； 5 µL (0.5 µg)/test（Flow）

产品详细信息Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: PE Mouse IgG1 K Isotype Control has been reported for use in flow cytometric analysis.Applications Tested: This Mouse IgG1 K Isotype Control is offered in 2 formats: - µg size: has been tested by flow cytometric analysis. Use isotype control at the same concentration as experimental antibody. - test size: has been pre-titrated and tested by flow cytometric analysis. This can be used at test size: 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.仅用于科研。不用于诊断过程。

数据

Mouse IgG1 kappa Isotype Control (12-4714-41) in FlowNormal human peripheral blood lymphocytes were stimulated with Cell Stimulation Cocktail plus protein transport inhibitors (Product # 00-4975) overnight. Cells were fixed and stained intracellularly with Anti-Human CD3 PerCP-Cyanine5.5 (Product # 45-0036-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-82) (left) or Anti-Human Amphiregulin PE (Product # 12-5370) (right) using the Intracellular Fixation and Permeabilization Buffers set (Product # 88-8824). Viable cells the lymphocyte gate, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866), were used for analysis.

推荐产品：

-Intracellular Fixation & Permeabilization Buffer Set（货号#88-8824）-Foxp3 / Transcription Factor Staining Buffer Set（货号#00-5523-00）-Flow Cytometry Staining Buffer（货号#00-4222-57/26）

参考文献：

1. Chinese medicineGlossogyne tenuifolia(Hsiang-ju) extract suppresses T cell activation by inhibiting activation of c-Jun N-terminal kinase."12-4714 was used as a Control in experiments to study how anti-inflammatory effects exerted by GTE are mediated via suppression of JNK phosphorylation in T cell activation."AuthorsHoung JY,Tai TS,Hsu SC,Hsu HF,Hwang TS,Lin CJ,Fang LWYear2020Dilution1:50 2. Frontiers in immunologyTGF-β Affects the Differentiation of Human GM-CSF+CD4+T Cells in an Activation- and Sodium-Dependent Manner."12-4714 was used in Flow cytometry/Cell sorting to study the environmental cues that promote differentiation of granulocyte-macrophage colony-stimulating factor producing T cells."AuthorsÉliás S,Schmidt A,Kannan V,Andersson J,Tegnér JYear2019 3. F1000ResearchA double blinded, placebo-controlled pilot study to examine reduction of CD34+/CD117+/CD133+lymphoma progenitor cells and duration of remission induced by neoadjuvant valspodar in dogs with large B-cell lymphoma."12-4714 was used in Flow cytometry/Cell sorting to examine whether ABCB1 would reduce lymphoma progenitor cells, and extend the length of remission in dogs with therapy naive large B-cell lymphoma."AuthorsIto D,Childress M,Mason N,Winter A,O'Brien T,Henson M,Borgatti A,Lewellen M,Krick E,Stewart J,Lahrman S,Rajwa B,Scott MC,Seelig D,Koopmeiners J,Ruetz S,Modiano JYear2019 4. Nature communicationsBioreactor-based mass production of human iPSC-derived macrophages enables immunotherapies against bacterial airway infections."12-4714 was used in Flow cytometry/Cell sorting to develop a novel, cell-based treatment strategy to target bacterial airway infections."AuthorsAckermann M,Kempf H,Hetzel M,Hesse C,Hashtchin AR,Brinkert K,Schott JW,Haake K,Kühnel MP,Glage S,Figueiredo C,Jonigk D,Sewald K,Schambach A,Wronski S,Moritz T,Martin U,Zweigerdt R,Munder A,Lachmann NYear2018 5. Journal of experimental and clinical cancer research : CRApigenin suppresses PD-L1 expression in melanoma and host dendritic cells to elicit synergistic therapeutic effects."12-4714 was used in Flow cytometry/Cell sorting to investigate the effects of the flavonoid apigenin on the PD-L1 expression and the tumourigenesis of melanoma."AuthorsXu L,Zhang Y,Tian K,Chen X,Zhang R,Mu X,Wu Y,Wang D,Wang S,Liu F,Wang T,Zhang J,Liu S,Zhang Y,Tu C,Liu H

技术参数

细胞类型 造血干/祖细胞

产品线 MethoCult™

适用物种 人

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-14462.htm