CD137 (4-1BB) Monoclonal Antibody (17B5), Functional Grade, eBioscience™/CD137 (4-1BB) 单克隆 (17B5), 功能抗体, eBioscience™

货号：16-1371-85,16-1371-38,16-1371-025,16-1371-050

规格：500 µg,5 mg,25 mg,50 mg

价格：3496,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD137

物种：小鼠

宿主：其它

抗体亚型：IgG

克隆号：17B5

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Syrian Hamster IgG Isotype Control, Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The 17B5 monoclonal antibody reacts with mouse CD137, also known as 4-1BB, a member of the TNFR superfamily. CD137 is expressed as monomers, dimers, and tetramers on the surface of activated T cells around day 4-6 after activation through the TCR complex. Interaction of CD137 with its ligand, 4-1BBL, is reported to deliver a costimulatory signal important in the T-APC interaction. Recent data suggests CD317 is expressed on Tregs and may play a role in their proliferation. The monoclonal antibody 17B5 has strong antagonistic effect in in vitro functional assays.Applications Reported: This 17B5 antibody has been reported for use in flow cytometric analysis and functional assays.Applications Tested: The 17B5 antibody has been tested by flow cytometric analysis of activated mouse splenocytes cultures. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.仅用于科研。不用于诊断过程。

数据

CD137 (4-1BB) Antibody (16-1371-85)Cell reports 2019 -Figure 2 Short Schedule of MEKi Treatment Alters T Cell Activation Status ExVivo (A) Schema of ex vivo short versus long treatmentexperiment. (B) CTLA-4, PD1, Ki-67, and 4-1BB expression in CD8+ T cells andCD4+Foxp3 cells by flow cytometry after selumetinib (left) or trametinib (right)treatment for 96 hr. *p < 0.05; **p < 0.01, Welch'stest. Error bars represent SD. Samples were biological replicates. Theexperiment was performed twice, and representative results are shown here.

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参考文献：

流式1.Cell reportsPulsatile MEK Inhibition Improves Anti-tumor Immunity and T Cell Function in Murine Kras Mutant Lung Cancer."Published figure using CD137 (4-1BB) monoclonal antibody (Product # 16-1371-85) in Flow Cytometry"2.Cancer immunology researchVaccination with High-Affinity Epitopes Impairs Antitumor Efficacy by Increasing PD-1 Expression on CD8+T Cells."Published figure using CD137 (4-1BB) monoclonal antibody (Product # 16-1371-85) in Flow Cytometry"3.Cancer researchAnti-PD-L1 Efficacy Can Be Enhanced by Inhibition of Myeloid-Derived Suppressor Cells with a Selective Inhibitor of PI3Kδ/γ."16-1371 was used in Flow cytometry/Cell sorting to demonstrate functional inhibition of myeloid-derived suppressor cells with IPI-145 which enhances responses to PD-L1 blockade."AuthorsDavis RJ,Moore EC,Clavijo PE,Friedman J,Cash H,Chen Z,Silvin C,Van Waes C,Allen C功能检测4.European journal of immunologyTNF optimally activatives regulatory T cells by inducing TNF receptor superfamily members TNFR2, 4-1BB and OX40."16-1371 was used in Functional assays to investigate pleiotropy in TNF inflammatory effects, showing that it activates Tregs by inducing TNFR2, 4-1BB and OX40."AuthorsHamano R,Huang J,Yoshimura T,Oppenheim JJ,Chen X5.Journal of immunology (Baltimore, Md. : 1950)The 4-1BB costimulation augments the proliferation of CD4+CD25+ regulatory T cells.AuthorsZheng G,Wang B,Chen A

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