CD80 (B7-1) Monoclonal Antibody (2D10.4), Functional Grade, eBioscience™/CD80 (B7-1) 单克隆 (2D10.4), 功能抗体, eBioscience™

货号：16-0809-81,16-0809-85,16-0809-025,16-0809-050

规格：50 µg,500 µg,25 mg,50 mg

价格：1283,3735,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD80

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：2D10.4

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1 µg/test（Flow）

产品详细信息Description: The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.Applications Reported: The 2D10.4 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 2D10.4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.仅用于科研。不用于诊断过程。

数据

CD80 (B7-1) Antibody (16-0809-81)Nutrients 2019 -Figure 1 Differential macrophage response to different inflammatory cytokine stimuli. ( A ) Morphological and immunocytochemical images showing monocyte-derived macrophage (MDM) polarization to M1 or M2 functional phenotype using type 1 cytokine IFN-gamma and lipopolysaccharide (LPS) or type 2 cytokine IL-4 and IL-13, respectively. ( B ) Increases in CD80+ M1 and CD206+ M2 MDMs were seen after treatment with IFN-gamma /LPS or IL-4/IL-13, respectively, using flow-cytometry analysis. Green arrows point to the CD80+ M1 macrophage, while red arrows indicate CD206+ M2 macrophages. ( C ) Flow cytometry and morphology imaging of M1 and M2 cell sorting and isolation using the fluorescence-activated cell sorting (FACS) assay. ( D ) Compared with the H1299 cells treated with M1 conditioned medium, the H1299 cells cultured with M2 conditioned medium exhibited higher invasion ability at 18 h in matrigel study. APC: antigen-presenting cell.

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参考文献：

流式1.NutrientsAstragalus polysaccharides(PG2) Enhances the M1 Polarization of Macrophages, Functional Maturation of Dendritic Cells, and T Cell-Mediated Anticancer Immune Responses in Patients with Lung Cancer."Published figure using CD80 (B7-1) monoclonal antibody (Product # 16-0809-81) in Flow Cytometry"2.Oncology lettersPhotodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70."Published figure using CD80 (B7-1) monoclonal antibody (Product # 16-0809-81) in Flow Cytometry"3.Journal of immunology (Baltimore, Md. : 1950)Endothelial microparticles interact with and support the proliferation of T cells."16-0809 was used in Flow cytometry/Cell sorting to explore the effect of endothelial cell microparticles in T cell activation and antigen presentation."AuthorsWheway J,Latham SL,Combes V,Grau GE4.Journal of immunology (Baltimore, Md. : 1950)Phagocytosis, a potential mechanism for myeloid-derived suppressor cell regulation of CD8+ T cell function mediated through programmed cell death-1 and programmed cell death-1 ligand interaction."16-0809 was used in Flow cytometry/Cell sorting to identify a potential mechanism by which IFN-γ-producing CD8(+) T cells are tolerised after type 1 immune responses to chronic virus or tumour."AuthorsKim YJ,Park SJ,Broxmeyer HE 功能检测5. The Journal of clinical investigationRenal allograft rejection is prevented by adoptive transfer of anergic T cells in nonhuman primates.AuthorsBashuda H,Kimikawa M,Seino K,Kato Y,Ono F,Shimizu A,Yagita H,Teraoka S,Okumura KIF5.OncoTargets and therapyA DNA vaccine encoding mutated HPV58 mE6E7-Fc-GPI fusion antigen and GM-CSF and B7.1."Published figure using CD80 (B7-1) monoclonal antibody (Product # 16-0809-81) in Immunofluorescence"

技术参数

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