MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Functional Grade, eBioscience™/MHC Class II (I-A/I-E) 单克隆 (M5/114.15.2), 功能抗体, eBioscience™

货号：16-5321-81,16-5321-85,16-5321-38,16-5321-025,16-5321-050

规格：50 µg,500 µg,5 mg,25 mg,50 mg

价格：1044,3847,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：MHC Class II

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：M5/114.15.2

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.125 µg/test（Flow）

产品详细信息Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.Applications Reported: M5/114.15.2 has been reported for use in flow cytometric analysis and in vitro blocking of T cell proliferative responses.Applications Tested: The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.仅用于科研。不用于诊断过程。

数据

MHC Class II (I-A/I-E) Antibody (16-5321-81) in FlowStaining of mouse splenocytes with Anti-Mouse MHC Class II (I-A/I-E) FITC (left) and APC (right).Appropriate isotype controls were used (open histogram).Total viable cells were used for analysis.

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参考文献：

流式1.American journal of translational researchThiol peroxiredoxin, a novel allergen fromBombyx mori, modulates functions of macrophages and dendritic cells."Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry"2.Journal for immunotherapy of cancerMDM2 inhibitor APG-115 synergizes with PD-1 blockade through enhancing antitumor immunity in the tumor microenvironment."Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry"3.Frontiers in immunologyProlonged Deleterious Influences of Chemotherapeutic Agent CPT-11 on Resident Peritoneal Macrophages and B1 Cells."Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry"WB4.The Journal of experimental medicineThe intramembrane protease SPPL2a promotes B cell development and controls endosomal traffic by cleavage of the invariant chain."16-5321 was used in Immuno-electron microscopy to investigate the role of SPPL2a in the cleavage of type 2 transmembrane proteins, showing that it promotes B cell development and controls endosomal traffic."AuthorsSchneppenheim J,Dressel R,Hüttl S,Lüllmann-Rauch R,Engelke M,Dittmann K,Wienands J,Eskelinen EL,Hermans-Borgmeyer I,Fluhrer R,Saftig P,Schröder BIHC5.Frontiers in cellular neuroscienceAllergy Enhances Neurogenesis and Modulates Microglial Activation in the Hippocampus."Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunofluorescence"

技术参数

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