CD80 (B7-1) Monoclonal Antibody (16-10A1), Functional Grade, eBioscience™/CD80 (B7-1) 单克隆 (16-10A1), 功能抗体, eBioscience™

货号：16-0801-82,16-0801-85,16-0801-025,16-0801-050

规格：100 µg,500 µg,25 mg,50 mg

价格：1865,3274,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD80

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：16-10A1

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.Applications Reported: The 16-10A1 antibody has been reported for use in flow cytometric analysis. 16-10A1 has also been reported in blocking of CD80 to its ligands.Applications Tested: The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.仅用于科研。不用于诊断过程。

数据

CD80 (B7-1) Antibody (16-0801-82) in FlowSurface staining of LPS stimulated splenocytes with Anti-Mouse CD80 (B7-1) FITC (left), and Anti-Mouse CD80 (B7-1) PE (right).Appropriate isotype controls were used (open histogram).Total viable cells were used for analysis.

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参考文献：

流式1.Frontiers in immunologyFcεRI γ-Chain Negatively Modulates Dectin-1 Responses in Dendritic Cells."Published figure using CD80 (B7-1) monoclonal antibody (Product # 16-0801-82) in Flow Cytometry"2.Nature communicationsT cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells."Published figure using CD80 (B7-1) monoclonal antibody (Product # 16-0801-82) in Flow Cytometry"3.Molecular medicine reportsDiscrimination of the heterogeneity of bone marrow‑derived dendritic cells."Published figure using CD80 (B7-1) monoclonal antibody (Product # 16-0801-82) in Flow Cytometry"功能检测4.Mediators of inflammationCD80 and CD86 costimulatory molecules differentially regulate OT-II CD4⁺ T lymphocyte proliferation and cytokine response in cocultures with antigen-presenting cells derived from pregnant and pseudopregnant mice."16-0801 was used in Functional assays to assess the effects of co-culturing of T cells with ovalbumin presenting macrophage and dendritic cells on proliferation, activation and cytokine profiles."AuthorsMaj T,Slawek A,Chelmonska-Soyta A中和5.American journal of respiratory and critical care medicineCD40 and CD80/86 act synergistically to regulate inflammation and mortality in polymicrobial sepsis."16-0801 was used in Blocking experiments to study CD40 and CD80/86 action in the regulation of inflammation and mortality in polymicrobial sepsis."AuthorsNolan A,Weiden M,Kelly A,Hoshino Y,Hoshino S,Mehta N,Gold JA6.BloodPreventing restimulation of memory B cells in hemophilia A: a potential new strategy for the treatment of antibody-dependent immune disorders."16-0801 was used in Blocking experiments to study the requirements for the restimulation of FVIII-specific memory B cells and their differentiation into anti-FVIII antibody-producing cells."AuthorsHausl C,Ahmad RU,Schwarz HP,Muchitsch EM,Turecek PL,Dorner F,Reipert BM

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