CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), Functional Grade, eBioscience™/CD274 (PD-L1, B7-H1) 单克隆 (MIH1), 功能抗体, eBioscience™

货号：16-5983-82,16-5983-203,16-5983-38,16-5983-025,16-5983-050

规格：100 µg,3 mg,5 mg,25 mg,50 mg

价格：3025,17569,24836,74506,124177

产品类型：流式抗体

品牌：eBioscience

抗原：CD274

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：MIH1

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1 µg/test（Flow）

产品详细信息Description: The MIH1 monoclonal antibody reacts with human B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH1 is reported to be a blocking antibody.Applications Reported: The MIH1 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking in in vitro functional assays.Applications Tested: The MIH1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.仅用于科研。不用于诊断过程。

数据

CD274 (PD-L1, B7-H1) Antibody (16-5983-82) in FlowStaining of normal human peripheral blood cells with Anti-Human CD274 (B7-H1) PE.Appropriate isotype controls were used (open histogram).Cells in the lymphocyte population were used for analysis.

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参考文献：

流式1.Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer AssociationEBV-associated gastric cancer evades T-cell immunity by PD-1/PD-L1 interactions."16-5983 was used in Flow cytometry/Cell sorting to evaluate the PD-L1 expression and function in Epstein-Barr virus-associated gastric cancer."AuthorsSasaki S,Nishikawa J,Sakai K,Iizasa H,Yoshiyama H,Yanagihara M,Shuto T,Shimokuri K,Kanda T,Suehiro Y,Yamasaki T,Sakaida I2.PLoS neglected tropical diseasesSimilarities and differences between helminth parasites and cancer cell lines in shaping human monocytes: Insights into parallel mechanisms of immune evasion."16-5983 was used in Flow cytometry/Cell sorting to suggest that despite the fact that helminth parasites and cancer cell lines are extraordinarily disparate, they share the ability to alter the phenotype of human monocytes."AuthorsNarasimhan PB,Akabas L,Tariq S,Huda N,Bennuru S,Sabzevari H,Hofmeister R,Nutman TB,Tolouei Semnani R功能检测3.OncoimmunologyPoly(I:C) primes primary human glioblastoma cells for an immune response invigorated by PD-L1 blockade."16-5983 was used in Functional assays to study the combination of poly(I:C) and PD-L1 blockade to invigorate the immune checkpoint inhibition response in glioblastoma."AuthorsDe Waele J,Marcq E,Van Audenaerde JR,Van Loenhout J,Deben C,Zwaenepoel K,Van de Kelft E,Van der Planken D,Menovsky T,Van den Bergh JM,Willemen Y,Pauwels P,Berneman ZN,Lardon F,Peeters M,Wouters A,Smits EL4.Journal of leukocyte biologyThe PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by α-PD-L1 or α-IL6 antibodies."16-5983 was used in Functional assays to study the effects of α-PD-L1 or α-IL6 antibodies in preventing the dampening of the IL27/STAT1 anticancer responses."AuthorsRolvering C,Zimmer AD,Ginolhac A,Margue C,Kirchmeyer M,Servais F,Hermanns HM,Hergovits S,Nazarov PV,Nicot N,Kreis S,Haan S,Behrmann I,Haan C中和5.OncotargetA versatile T cell-based assay to assess therapeutic antigen-specific PD-1-targeted approaches."16-5983 was used in Neutralization experiments to develop an antigen-specific, short-term and high-throughput T cell assay with versatile readout possibilities."AuthorsVersteven M,Van den Bergh JMJ,Broos K,Fujiki F,Campillo-Davo D,De Reu H,Morimoto S,Lecocq Q,Keyaerts M,Berneman Z,Sugiyama H,Van Tendeloo VFI,Breckpot K,Lion E

技术参数

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