CD275 (B7-H2) Monoclonal Antibody (HK5.3), Functional Grade, eBioscience™/CD275 (B7-H2) 单克隆 (HK5.3), 功能抗体, eBioscience™

货号：16-5985-85,16-5985-025,16-5985-050

规格：500 µg,25 mg,50 mg

价格：4667,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD275

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：HK5.3

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The HK5.3 monoclonal antibody reacts with mouse B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of ~40 kDa and belongs to the Ig superfamily. Mouse B7RP-1 is expressed by antigen presenting cells including B cells, monocytes/macrophages and dendritic cells. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway.Applications Reported: The HK5.3 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking in functional assays.Applications Tested: The HK5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS). ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function. ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue. Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.仅用于科研。不用于诊断过程。

数据

CD275 (B7-H2) Antibody (16-5985-85) in FlowStaining of mouse splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and Anti-Mouse CD275 (B7-H2) PE.Total viable cells were used for analysis.

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参考文献：

流式1.OncotargetLysis-independent potentiation of immune checkpoint blockade by oncolytic virus."Published figure using CD275 (B7-H2) monoclonal antibody (Product # 16-5985-85) in Flow Cytometry"中和2.BloodPreventing restimulation of memory B cells in hemophilia A: a potential new strategy for the treatment of antibody-dependent immune disorders."16-5985 was used in Blocking experiments to study the requirements for the restimulation of FVIII-specific memory B cells and their differentiation into anti-FVIII antibody-producing cells."AuthorsHausl C,Ahmad RU,Schwarz HP,Muchitsch EM,Turecek PL,Dorner F,Reipert BM

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