North2South Complete Biotin Random Prime Labeling and Detection Kit
货号：17175
规格：10 blots
价格：9617
产品类型：核酸标记
品牌：Thermo Fisher
| The Thermo Scientific North2South Biotin Random Prime Labeling Kit provides the components and procedure to successfully prepare biotin-labeled DNA probes for use in Southern blotting and other DNA hybridization methods involving streptavidin detection systems.This kit includes the North2South Chemiluminescent Detection Kit, which combines a novel ECL substrate for horseradish peroxidase (HRP) and optimized hybridization and blocking buffers to ensure consistent, high-sensitivity Southern blot results.The North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin- HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera. |
| 特点 |
| • Sensitivity equal to or greater than32P• Much faster than DIG detection systems• Amenable to nearly any DNA or RNA hybridization method• Optimized hybridization and blocking buffers and protocol steps yield consistent results from experiment to experiment• High level of light output and consistent low background allows for very short and multiple exposure times using cooled CCD camera systems or film• Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user• Specific plaques can be picked out of areas that are very dense with signal due to extremely low background |
技术参数 组成成分 Sufficient For: 10 biotin-labeling reactions (each with 100 ng DNA) and 10 mini-gel blots• North2South Chemiluminescent Hybridization and Detection Kit (Part No. 17097), 1 kit• North2South Biotin Random Prime Labeling Kit (Part No. 17075), 1 kit
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-26391.htm
QQ:1749072012