Mouse anti Human MCM7 antibody
货号：VMA00544
规格：100μl
价格：2916
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：MCM7
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：AB04/3F1
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Human MCM7 antibodyrecognizes the DNA replication licensing factor MCM7, also known as CDC47 homolog, homolog of S. cerevisiae Cdc47, minichromosome maintenance deficient 7 or protein phosphatase 1, regulatory subunit 104.The protein encoded by MCM7 gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported (provided by RefSeq, Jul 2008).Mouse anti Human MCM7 antibody detects a band of 81 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3). |
| Immunogen | E. coli-derived recombinant human MCM7 (amino acids 1 - 390) |
| Approx. Protein Concentrations | IgG concentration 0.5 mg/ml. |
| Acknowledgements | PrecisionAb is a trademark of Bio-Rad Laboratories. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch. |
| 存储条件 | | |
| Store undiluted at -20°C, avoiding repeated freeze thaw cycles. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 1/1000 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood. |
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技术参数 产品应用 wb
产品类型 单克隆抗体
推荐用于 wb
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