CD273 (B7-DC) Monoclonal Antibody (MIH18), Functional Grade, eBioscience™/CD273 (B7-DC) 单克隆 (MIH18), 功能抗体, eBioscience™

货号：16-5888-82,16-5888-86,16-5888-38,16-5888-025,16-5888-050

规格：100 µg,1 mg,5 mg,25 mg,50 mg

价格：3386,99999999999,999999999999,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD273

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：MIH18

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The MIH18 monoclonal antibody reacts with human B7-DC, also known as PD-L2. B7-DC, a recently identified member of the B7 family, has a predicted molecular weight of approximately 25 kDa and belongs to the Ig superfamily. B7-DC is primarily expressed by subpopulations of dendritic cells and monocytes/macrophages. Although B7-DC has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, rather it is a ligand for PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation. MIH18 is a useful tool for studies to elucidate the exact function of B7-DC/PD-L2 in APC/T cell interaction and to characterize the expression pattern of this molecule in humans. MIH18 is a blocking antibody.Applications Reported: This MIH18 antibody has been reported for use in flow cytometric analysis and functional assays.Applications Tested: The MIH18 antibody has been tested by flow cytometric analysis of mature dendritic cells and normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. [UniProt]仅用于科研。不用于诊断过程。。

数据

CD273 (B7-DC) Antibody (16-5888-82) in FlowStaining of in vitro derived human dendritic cells with 0.25 µg of Mouse IgG1 Isotype Control Functional Grade Purified (Product # 16-4714-82) (open histogram) or 0.25 µg of Anti-Human CD273 (B7-DC) Functional Grade Purified followed by Anti-Mouse IgG Biotin (Product # 13-4013-85) and Streptavidin PE (Product # 12-4317-87) (filled histogram). Total viable cells were used for analysis.

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参考文献：

流式1.Journal of hematology and oncologyPD-1 axis expression in musculoskeletal tumors and antitumor effect of nivolumab in osteosarcoma model of humanized mouse."Published figure using CD273 (B7-DC) monoclonal antibody (Product # 16-5888-82) in Flow Cytometry"2.Immunology lettersCytokine induced expression of programmed death ligands in human neutrophils."16-5888 was used in Flow cytometry/Cell sorting to reveal a possible immunoregulatory role for human neutrophils in adaptive immunity."AuthorsBankey PE,Banerjee S,Zucchiatti A,De M,Sleem RW,Lin CF,Miller-Graziano CL,De AK功能检测3.OncoimmunologyPoly(I:C) primes primary human glioblastoma cells for an immune response invigorated by PD-L1 blockade."16-5888 was used in Functional assays to study the combination of poly(I:C) and PD-L1 blockade to invigorate the immune checkpoint inhibition response in glioblastoma."AuthorsDe Waele J,Marcq E,Van Audenaerde JR,Van Loenhout J,Deben C,Zwaenepoel K,Van de Kelft E,Van der Planken D,Menovsky T,Van den Bergh JM,Willemen Y,Pauwels P,Berneman ZN,Lardon F,Peeters M,Wouters A,Smits EL4.Journal of immunology (Baltimore, Md. : 1950)PD-1 is a regulator of NY-ESO-1-specific CD8+ T cell expansion in melanoma patients."16-5888 was used in Functional assays to investigate the role of PD-1 in impairing spontaneous tumour Ag-specific CD8(+) T cells in melanoma patients."IHC5.Investigative ophthalmology and visual sciencePD-L1 expression on human ocular cells and its possible role in regulating immune-mediated ocular inflammation."16-5888 was used in Immunohistochemistry to show that the expression of PD-L1 on human ocular cells inhibits the production of proinflammatory cytokines and a Th2 cytokine."AuthorsYang W,Li H,Chen PW,Alizadeh H,He Y,Hogan RN,Niederkorn JY

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