CD70 Monoclonal Antibody (FR70), Functional Grade, eBioscience™/CD70 单克隆 (FR70), 功能抗体, eBioscience™

货号：16-0701-82,16-0701-81,16-0701-85,16-0701-38,16-0701-025,16-0701-050

规格：100 µg,50 µg,500 µg,5 mg,25 mg,50 mg

价格：1752,1044,3496,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD70

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：FR70

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The FR70 monoclonal antibody reacts with mouse CD70, a 30-33 kDa type II transmembrane glycoprotein. CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes. Interaction of CD70 with CD27, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.Applications Reported: The FR70 antibody has been reported for use in flow cytometric analysis. FR70 has also been reported in blocking of CD70 in vitro .Applications Tested: The FR70 antibody has been tested by flow cytometric analysis of LPS-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis.仅用于科研。不用于诊断过程。

数据

CD70 Antibody (16-0701-82) in FlowStaining of 4-day LPS activated BALB/c splenocytes with 0.25 µg of Rat IgG2b kappa Isotype Control Functional Grade Purified (Product # 16-4031-81) (open histogram) or 0.25 µg of Anti-Mouse CD70 Functional Grade Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85)and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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参考文献：

流式1.BloodLymphatic endothelial cells induce tolerance via PD-L1 and lack of costimulation leading to high-level PD-1 expression on CD8 T cells."16-0701 was used in Flow cytometry/Cell sorting to demonstrate that LECs mediate deletion only via programmed cell death-1 (PD-1) ligand 1."AuthorsTewalt EF,Cohen JN,Rouhani SJ,Guidi CJ,Qiao H,Fahl SP,Conaway MR,Bender TP,Tung KS,Vella AT,Adler AJ,Chen L,Engelhard VH2.Journal of immunology (Baltimore, Md. : 1950)CD70-driven costimulation induces survival or Fas-mediated apoptosis of T cells depending on antigenic load."16-0701 was used in Flow cytometry/Cell sorting to investigate how BIm-dependent apoptosis is initiated in CD8 T cells."AuthorsWensveen FM,Unger PP,Kragten NA,Derks IA,ten Brinke A,Arens R,van Lier RA,Eldering E,van Gisbergen KP3.Nature immunologyCostimulation via the tumor-necrosis factor receptor superfamily couples TCR signal strength to the thymic differentiation of regulatory T cells."16-0701 was used in Flow cytometry/Cell sorting to study the role of TNF receptors in the thymic development of Treg cells.."AuthorsMahmud SA,Manlove LS,Schmitz HM,Xing Y,Wang Y,Owen DL,Schenkel JM,Boomer JS,Green JM,Yagita H,Chi H,Hogquist KA,Farrar MA功能检测4.ImmunityEpitope-specific regulation of memory programming by differential duration of antigen presentation to influenza-specific CD8(+) T cells."16-0701 was used in funtional assays to characterise the signals received by CD8 T cells responding to different epitopes of influenza virus during the primary response, and the effects on their secondary responsiveness."AuthorsBallesteros-Tato A,León B,Lee BO,Lund FE,Randall TD5.Proceedings of the National Academy of Sciences of the United States of AmericaLoss of T cell receptor-induced Bmi-1 in the KLRG1(+) senescent CD8(+) T lymphocyte."16-0701 was used in Immunopurification to indicate that Bmi-1 is required for CD8(+) T cell clonal expansion and is positively regulated by receptors that mediate this response."AuthorsHeffner M,Fearon DT

技术参数

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