IL-2 Monoclonal Antibody (JES6-5H4), Functional Grade, eBioscience™/IL-2 单克隆 (JES6-5H4), 功能抗体, eBioscience™

货号：16-7021-85,16-7021-38,16-7021-025,16-7021-050

规格：500 µg,5 mg ,25 mg,50 mg

价格：3386,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：IL-2

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：JES6-5H4

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5-2 µg/mL（ELISA）

产品详细信息Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.Applications Reported: The functional grade purified JES6-5H4 has been reported for use in blocking of fluorochrome conjugated JES6-5H4 in intracellular flow cytometry experiments to serve as specificity control. JES6-5H4 is reported for detection of mouse IL-2 by ELISA, immunoprecipitation, and for neutralizing IL-2 binding and biological activity.Applications Tested: The JES6-5H4 antibody has been tested by sandwich ELISA for detection of mouse IL-2 in combination with JES6-1A12 and can be used between 0.5-2 µg/mL.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.仅用于科研。不用于诊断过程。

数据

IL-2 Antibody (16-7021-85)Intracellular staining of stimulated mouse splenocytes. As expected based on known expression patterns, IL-2 clone JES6-5H4 stains a large subset of CD4+ T cells and a minor subset of NK1.1+ NK cells with no staining observed without stimulation. Details: Mouse splenocytes were cultured in the presence of Protein Transport Inhibitors (500X) (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation and Permeabilization Buffer Set and protocol followed by intracellular staining with CD4 (clone RM4-5), NK1.1 (clone PK136) and IL-2 (clone JES6-5H4). Cells in the CD4+ (blue histogram) or NK1.1+ (purple histogram) gates were used for analysis.

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参考文献：

流式1.EBioMedicineA Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation."Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry"2.Human vaccines and immunotherapeuticsImmunogenicity and protective efficacy of DMT liposome-adjuvanted tuberculosis subunit CTT3H vaccine."Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry"3.Frontiers in immunologyA New Adjuvant MTOM MediatesMycobacterium tuberculosisSubunit Vaccine to Enhance Th1-Type T Cell Immune Responses and IL-2+T Cells."Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry"4.BloodEnhanced efficacy and reduced toxicity of multifactorial adjuvants compared with unitary adjuvants as cancer vaccines."16-7021 was used in Flow cytometry/Cell sorting to report the therapeutic effects of combinatorial use of CD40 and TLR agonists as a cancer vaccine."AuthorsAhonen CL,Wasiuk A,Fuse S,Turk MJ,Ernstoff MS,Suriawinata AA,Gorham JD,Kedl RM,Usherwood EJ,Noelle R5.VaccineEnvironmental effects on protection against Mycobacterium tuberculosis after immunization with Ad85A."16-7021 was used in Flow cytometry/Cell sorting to show that exposure to environmental microorganisms can alter the protective immune response to a parenterally administered subunit vaccine."AuthorsBeverley P,Ronan E,Lee L,Arnold I,Bolinger B,Powrie F,Tchilian E

技术参数

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