IL-10 Monoclonal Antibody (JES3-9D7), Functional Grade, eBioscience™/IL-10 单克隆 (JES3-9D7),功能抗体, eBioscience™

货号：16-7108-81,16-7108-85,16-7108-38,16-7108-025,16-7108-050

规格：50 µg,500 µg,5 mg,25 mg,50 mg

价格：1156,3386,19872,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：IL-10

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG1, kappa

克隆号：JES3-9D7

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	Assay-Dependent（ELISA）;Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).Applications Reported: The JES3-9D7 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization, and intracellular staining for flow cytometric analysis.Applications Tested: The JES3-9D7 antibody has been tested for neutralizing IL-10 binding and biological activity.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturingfiltered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.仅用于科研。不用于诊断过程。

数据

IL-10 Antibody (16-7108-81)mSystems 2020 -FIG 2 Spore-forming bacteria from MS patients inhibit IL-10 + Treg differentiation in vitro . (A and B) Representative flow cytometry plots (A) and quantification (B) of CD4 + FoxP3 + Tregs within CD3 + lymphocytes differentiated in response to spore-forming bacteria isolated from controls or untreated MS patients. n = 7 PBMC donors; each dot represents an average response from PBMC donor to isolates from 6 control or MS bacterial donors. * * , P < 0.01, two-tailed repeated measures t test. (C and D) Representative flow cytometry plots (C) and quantification (D) of IL-10 + lymphocyte population within CD3 + CD4 + FoxP3 + Tregs differentiated in response to spore-forming bacteria isolated from controls or untreated MS patients. n = 6 bacterial donors per group. * , P < 0.05, two-tailed t test. Error bars, mean +- SEM. The experiment was repeated with nonoverlapping PBMC and bacterial donors and gave the same results. (E) Quantification of T effector cell proliferation in response to Tregs differentiated in the presence of spore-forming bacteria from MS patients or controls. n = 3 bacterial donors per group, each representing an average of 3 technical replicates. (F) Linear correlation between IL-10 + population within CD3 + CD4 + FoxP3 + Tregs and Clostridia-Bacilli relative abundances. R 2 = 0.214, P = 0.0459. Black dots, MS patients. Light gray dots, controls.

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参考文献：

流式1.mSystemsMultiple Sclerosis-Associated Changes in the Composition and Immune Functions of Spore-Forming Bacteria."Published figure using IL-10 monoclonal antibody (Product # 16-7108-81) in Flow Cytometry"2.OncotargetDescription of organ-specific phenotype, and functional characteristics of tissue resident lymphocytes from liver transplantation donor and research on immune tolerance mechanism of liver."16-7108 was used in Flow cytometry to study the phenotype and function of tissue resident lymphocytes in peripheral blood, lymph node, spleen and liver."AuthorsShi Y,Zhang P,Wang G,Liu X,Sun X,Zhang X,Li H,Qi J,Ding L,Li T,Zhang R,Chen Y,Zhou J,Lv G,Tu Z功能检测3.Journal of leukocyte biologyBacterial reprogramming of PBMCs impairs monocyte phagocytosis and modulates adaptive T cell responses."16-7108 was used in Functional assays to characterise bacterially reprogrammed peripheral blood mononuclear cell-derived monocytes, with specific focus on their phagocytic activity."AuthorsAndré MC,Gille C,Glemser P,Woiterski J,Hsu HY,Spring B,Keppeler H,Kramer BW,Handgretinger R,Poets CF,Lauber K,Orlikowsky TW4.Journal of immunology (Baltimore, Md. : 1950)Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S.AuthorsSendide K,Deghmane AE,Pechkovsky D,Av-Gay Y,Talal A,Hmama Z中和5.Oncology lettersThe regulation of cancer cell migration by lung cancer cell-derived exosomes through TGF-β and IL-10."16-7108 was used in Blocking experiments to investigate the role of exosomes in tumourigenesis."6.Journal of immunology (Baltimore, Md. : 1950)The role of JAK-3 in regulating TLR-mediated inflammatory cytokine production in innate immune cells."16-7108 was used in Neutralization experiments to demonstrate the signaling pathway by which JAK3 differentially regulates TLR-mediated inflammatory cytokine production in innate immune cells."AuthorsWang H,Brown J,Gao S,Liang S,Jotwani R,Zhou H,Suttles J,Scott DA,Lamont RJ

技术参数

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