IL-10 Monoclonal Antibody (JES5-16E3), Functional Grade, eBioscience™/IL-10 单克隆 (JES5-16E3), 功能抗体, eBioscience™

货号：16-7101-81,16-7101-85,16-7101-38,16-7101-050,16-7101-025

规格：50 µg,500 µg,5 mg,50 mg,25 mg

价格：1044,3274,19872,120443,72266

产品类型：流式抗体

品牌：eBioscience

抗原：IL-10

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：JES5-16E3

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1-4 µg/mL（ELISA）;Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). Mouse IL-10 is an ~18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.Applications Reported: The JES5-16E3 antibody has been reported for use in capture of mouse IL-10 by ELISA and ELISPOT, intracellular staining for flow cytometric analysis, IHC, and neutralization of IL-10 bioactivity.Applications Tested: The Functional Grade purified JES5-16E3 antibody has been tested for ELISPOT capture. A suitable range of concentrations of this antibody for ELISPOT capture is 1-4 µg/mL.The JES5-16E3 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-10 (IL-10) in combination with the biotinylated JES5-2A5 (13-7102) antibody for detection and recombinant mouse IL-10 (14-8101) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/mL - 30 pg/mL should be included in each ELISA plate.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.仅用于科研。不用于诊断过程。

数据

IL-10 Antibody (16-7101-81) in ELISAMouse splenocytes (500,000 cells per well) were activated with LPS (1 µg/mL) for 48 hrs in Mouse IL-10 ELISPOT assay. Control well is medium alone.

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参考文献：

流式1.PloS oneRole of arginase 1 from myeloid cells in th2-dominated lung inflammation."16-7101 was used in Flow cytometry/Cell sorting to investigate the contribution of arginase 1 to asthma pathogenesis."AuthorsBarron L,Smith AM,El Kasmi KC,Qualls JE,Huang X,Cheever A,Borthwick LA,Wilson MS,Murray PJ,Wynn TA2.Journal of immunology (Baltimore, Md. : 1950)Canonical wnt signaling in dendritic cells regulates Th1/Th17 responses and suppresses autoimmune neuroinflammation."16-7101 was used in Flow cytometry/Cell sorting to study the factors in the tissue microenvironment and signalling networks critical for programming dendritic cells to control chronic inflammation and promote tolerance, showing that Th1/Th17 responses are regulated by canonical Wnt signalling in dendritic cells and suppresses autoimmune neuroinflammation."AuthorsSuryawanshi A,Manoharan I,Hong Y,Swafford D,Majumdar T,Taketo MM,Manicassamy B,Koni PA,Thangaraju M,Sun Z,Mellor AL,Munn DH,Manicassamy S功能检测3.Journal of translational medicineComparative analysis of cancer vaccine settings for the selection of an effective protocol in mice."16-7101 was used in a Functional assay to compare different gp100 vaccination strategies to identify the best strategy to provide a 100% protection against experimental melanoma in a reproducible manner."AuthorsKalli F,Machiorlatti R,Battaglia F,Parodi A,Conteduca G,Ferrera F,Proietti M,Tardito S,Sanguineti M,Millo E,Fenoglio D,De Palma R,Inghirami G,Filaci G中和4.EMBO molecular medicineTGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB."16-7101 was used in Flow cytometry/Cell sorting to investigate the role that regulatory T cells (in tumour-draining lymph nodes) play in modulating the malignancy of breast cancer cells for distant metastasis."5.Journal of neuroinflammationNOX2 deficiency alters macrophage phenotype through an IL-10/STAT3 dependent mechanism: implications for traumatic brain injury."16-7101 was used in Neutralization experiments to investigate the signalling pathways that regulate NOX2-dependent macrophage/microglial phenotype switching in the TBI brain."AuthorsBarrett JP,Henry RJ,Villapol S,Stoica BA,Kumar A,Burns MP,Faden AI,Loane DJ

技术参数

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