CD178 (Fas Ligand) Monoclonal Antibody (MFL3), Functional Grade, eBioscience™/CD178 (Fas Ligand) 单克隆 (MFL3), 功能抗体, eBioscience™

货号： 16-5911-82,16-5911-85,16-5911-38,16-5911-025,16-5911-050

规格：100 µg,500 µg,5 mg,25 mg,50 mg

价格：1992,4085,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD178

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：MFL3

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）;Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The MFL3 monoclonal antibody reacts with mouse Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by mouse activated T cells. The interaction of FasL with its receptor CD95 induces Fas-mediated killing. It has been reported that the human FasL antigen is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. The degree of sensitivity for the mouse antigen to MMPs has not beenreported.Applications Reported: MFL3 has been reported for use in flow cytometric analysis. It has also been reported in blocking of the FasL mediated killing in functional assays.Applications Tested: The MFL3 antibody has been tested by flow cytometric analysis of mouse FasL transfected cells and activated T cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD178 (Fas ligand, FasL) is a type-II-membrane protein, whose N-terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor, FasR, is a cell-surface-type-I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family. As a member of the TNF-cytokine family CD178 induces apoptosis when interacting with FasR. CD178 may exist as either membrane bound (45 kD) or soluble forms (26 kD). The soluble proteincan be released from cells upon cleavage by metalloproteinases. Binding of CD178 to Fas leads to oligomerization of the receptor and triggers apoptotic cell death through the interaction of other proteins. CD178 is predominantly expressed in activated T-lymphocytes and natural killer (NK) cells also it is expressed in the tissues of immune-privilege sites such as testis and eye. CD178 expression is also reported in various tissues as thymus, liver, ovary, lung, heart and kidney. It is assumed that induction of apoptosis through CD178 is predominantly involved in anti-viral immune responses. CD178 is a cell surface molecule belonging to the tumor necrosis factor family, binds to its receptor Fas, thus inducing apoptosis. Various cells express FAS, where CD178 is expressed predominantly on activated T cells. FAS and CD178 are involved in the down-regulation of immune reactions as well as T cell-mediated cytotoxicity. CD178 concentration has also been shown to be associated with atherosclerosis and inflammatory disease, in patients with hypertension. The Fas/ CD178 system has been shown to play a role in a number of human diseases, for example AIDS, hepatitis or cancer.仅用于科研。不用于诊断过程。

数据

CD178 (Fas Ligand) Antibody (16-5911-82) in FlowStaining of mouse Fas Ligand transfected cells with Anti-Mouse CD178 (Fas Ligand) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

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参考文献：

流式1.Nature communicationsExtrafollicular CD4+T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease."Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 16-5911-82) in Flow Cytometry"2.Journal of the American Society of Nephrology : JASNHypoxia-Inducible Factor-2α Limits Natural Killer T Cell Cytotoxicity in Renal Ischemia/Reperfusion Injury.功能检测3. Journal of the American Society of Nephrology : JASNHypoxia-Inducible Factor-2α Limits Natural Killer T Cell Cytotoxicity in Renal Ischemia/Reperfusion Injury.4.Cell death and diseaseApoptotic CD8 T-lymphocytes disable macrophage-mediated immunity to Trypanosoma cruzi infection."16-5911 was used in Functional assays to demonstrate that disposal of apoptotic CD8 T cells increases M2-macrophage differentiation and contributes to parasite persistence."AuthorsCabral-Piccin MP,Guillermo LV,Vellozo NS,Filardy AA,Pereira-Marques ST,Rigoni TS,Pereira-Manfro WF,DosReis GA,Lopes MF5.Frontiers in immunologyThioreductase-Containing Epitopes Inhibit the Development of Type 1 Diabetes in the NOD Mouse Model."16-5911 was used in Functional assays to develop a methodology by which CD4 T cells acquire apoptosis-inducing properties on antigen-presenting cells following cognate recognition of natural sequence epitopes."AuthorsMalek Abrahimians E,Vander Elst L,Carlier VA,Saint-Remy JM阻断6.Journal of immunology (Baltimore, Md. : 1950)NKG2C/E Marks the Unique Cytotoxic CD4 T Cell Subset, ThCTL, Generated by Influenza Infection."16591182 was used in blocking or activating experiment to determine that NKG2C/E identifies the CD4 T cell effector subset ThCTL that develop in the lung during influenza A virus infection in mice"AuthorsMarshall NB,Vong AM,Devarajan P,Brauner MD,Kuang Y,Nayar R,Schutten EA,Castonguay CH,Berg LJ,Nutt

技术参数

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