CD226 (DNAM-1) Monoclonal Antibody (10E5), Functional Grade, eBioscience™/CD226 (DNAM-1) 单克隆 (10E5), 功能抗体, eBioscience™
货号：16-2261-81,16-2261-85,16-2261-38,16-2261-025
规格：50 µg,500 µg,5 mg,25 mg
价格：1813,4600,24836,74506
产品类型：流式抗体
品牌：eBioscience
抗原：CD226
物种：小鼠
宿主：大鼠
抗体亚型：IgG2b, kappa
克隆号：10E5
荧光染料：其它
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类型: | 一抗(功能抗体） | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™ |
浓度： | 1 mg/mL | 用法： | 0.5 µg/test（Flow）;Assay-Dependent（功能检测） |
产品详细信息Description: This 10E5 monoclonal antibody reacts with mouse CD226, which is also known as DNAX accessory molecule-1 (DNAM-1). CD226 is an adhesion molecule expressed primarily on T cells, including thymocytes. The highest levels of CD226 are found on naive CD8 T cells. Moreover, expression of CD226 is regulated differentially in CD4 T cells. Under certain culture conditions, CD226 can be upregulated in Th1 and downregulated in Th2 and Th0 cells. This cell surface receptor can also be detected on natural killer cells, some B cell subsets, monocytes, and platelets. Interaction with its ligands, the poliovirus receptor (CD155) and CD112, results in adhesion and cell signaling events that promote innate and adaptive immunity, including differentiation, survival, and cytotoxicity.The 10E5 monoclonal antibody has been reported to inhibit antigen-specific T cell proliferation and IFN-gamma production in vivo.Applications Reported: This 10E5 antibody has been reported for use in flow cytometric analysis and functional assays.Applications Tested: This 10E5 antibody has been tested by flow cytometric analysis of 3-day CD3/CD28-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息DNAM 1 is a receptor expressed by peripheral blood T lymphocytes that is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T lymphocytes. DNAM 1 is broadly expressed on T cells, NK cells, platelets, monocytes and a subset of B cells. DNAM-1 is also expressed by a subset of CD3 positive thymocytes.仅用于科研。不用于诊断过程。
数据 |
| CD226 (DNAM-1) Antibody (16-2261-81) in FlowStaining of 3-day Anti-Mouse CD3/CD28-stimulated C57Bl/6 splenocytes with 0.25 µg of Rat IgG2b Kisotype Control Functional Grade Purified (Product # 16-4031-81) (left) or 0.25 µg of Anti-Mouse CD226 Functional Grade Purified (right) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87) and subsequently with Anti-Mouse CD8a FITC (Product # 11-0081-82).Total viable cells were used for analysis. |
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参考文献： |
| 流式1.Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacologyBlocking CD226 Promotes Allogeneic Transplant Immune Tolerance and Improves Skin Graft Survival by Increasing the Frequency of Regulatory T Cells in a Murine Model.AuthorsLiu T,Zhang D,Zhang Y,Xu X,Zhou B,Fang L,Zhang Y,Su Y,Jin B,Zhuang R,Guo S |
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