CD178 (Fas Ligand) Monoclonal Antibody (NOK-1), Functional Grade, eBioscience™/CD178 (Fas Ligand) 单克隆 (NOK-1), 功能抗体, eBioscience™

货号：16-9919-81,16-9919-85,16-9919-38,16-9919-025,16-9919-050

规格：50 µg,500 µg,5 mg,25 mg,50 mmg

价格：1283,3847,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD178

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：NOK-1

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1 µg/test（Flow）;Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The NOK-1 monoclonal antibody reacts with human Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by neutrophils, monocytes, and activated T cells and NK cells. The interaction of FasL with its receptor (CD95, Fas) induces Fas-mediated killing of lymphocytes. Human FasL is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. Therefore for optimal detection of surface FasL on activated peripheral blood cells, incubation of cells with an MMP inhibitor is recommended.Applications Reported: NOK-1 has been reported for use in flow cytometric analysis. NOK-1 has also been reported in blocking of FasL mediated killing in functional assays.Applications Tested: The NOK-1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD178 (Fas ligand, FasL) is a type-II-membrane protein, whose N-terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor, FasR, is a cell-surface-type-I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family. As a member of the TNF-cytokine family CD178 induces apoptosis when interacting with FasR. CD178 may exist as either membrane bound (45 kD) or soluble forms (26 kD). The soluble proteincan be released from cells upon cleavage by metalloproteinases. Binding of CD178 to Fas leads to oligomerization of the receptor and triggers apoptotic cell death through the interaction of other proteins. CD178 is predominantly expressed in activated T-lymphocytes and natural killer (NK) cells also it is expressed in the tissues of immune-privilege sites such as testis and eye. CD178 expression is also reported in various tissues as thymus, liver, ovary, lung, heart and kidney. It is assumed that induction of apoptosis through CD178 is predominantly involved in anti-viral immune responses. CD178 is a cell surface molecule belonging to the tumor necrosis factor family, binds to its receptor Fas, thus inducing apoptosis. Various cells express FAS, where CD178 is expressed predominantly on activated T cells. FAS and CD178 are involved in the down-regulation of immune reactions as well as T cell-mediated cytotoxicity. CD178 concentration has also been shown to be associated with atherosclerosis and inflammatory disease, in patients with hypertension. The Fas/ CD178 system has been shown to play a role in a number of human diseases, for example AIDS, hepatitis or cancer.仅用于科研。不用于诊断过程。

数据

CD178 (Fas Ligand) Antibody (16-9919-81) in FlowStaining of human FasL tranfected cells with Anti-Human CD178 (CD95 Ligand) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

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参考文献：

流式1.Scientific reportsFunctional dichotomy of Vδ2 γδ T cells in chronic hepatitis C virus infections: role in cytotoxicity but not for IFN-γ production."16-9919 was used in Flow cytometry/Cell sorting to analyse the phenotype and function of Vδ2 T cells in HCV-infected patients compared to healthy controls."AuthorsYin W,Tong S,Zhang Q,Shao J,Liu Q,Peng H,Hu H,Peng M,Hu P,Ren H,Tian Z,Zhang D2.International immunologyThe probiotic Escherichia coli strain Nissle 1917 induces gammadelta T cell apoptosis via caspase- and FasL-dependent pathways."16-9919 was used in Flow cytometry/Cell sorting to demonstrate E. coli Nissle profoundly regulates gammadelta T cell function."AuthorsGuzy C,Paclik D,Schirbel A,Sonnenborn U,Wiedenmann B,Sturm A3.OncotargetTransmembrane TNF-α promotes activation-induced cell death by forward and reverse signaling."Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 16-9919-81) in Flow Cytometry"

技术参数

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