CD305 (LAIR1) Monoclonal Antibody (113), Functional Grade, eBioscience™/CD305 (LAIR1) 单克隆 (113), 功能抗体, eBioscience™

货号：16-3051-82,16-3051-025,16-3051-050

规格：100 µg,25 mg,50 mg

价格：1752,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD305

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：113

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.25 µg/test（Flow）；Assay-Dependent（IP）；Assay-Dependent（FN）

产品详细信息Description: CD305 or Leukocyte-associated Ig-like receptor-1 (LAIR-1) is a 46 kD cell surface molecule that functions as an inhibitory collagen-receptor on the majority of cells of the immune system. The mouse LAIR-1 molecule is expressed primarily on monocytes/macrophages and also on T cells, NK cells and dendritic cells. Unlike human B cells , mouse B cells do not express LAIR-1. The cytoplasmic tail of mouse LAIR-1 can be phosphorylated leading to the recruitment of SH2-containing tyrosine phosphatase(SHP)-2 and C-terminal Src kinase.Applications Reported: This 113 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB). It has also been reported for use in functional studies.Applications Tested: This 113 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息LAIR1 (CD305) functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells,down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.仅用于科研。不用于诊断过程。

数据

CD305 (LAIR1) Antibody (16-3051-82) in FlowStaining of C57Bl/6 splenocytes with 0.125 µg of Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (purple line) or 0.125 µg of Anti-Mouse CD305 (LAIR-1) (blue line) followed by Anti-Armenian Hamster IgG PE (Product # 12-4112-83).Total viable F4/80 positive cells were used for analysis.

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参考文献：

流式1.PLoS pathogensHTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors."16-3051 was used in Flow cytometry/Cell sorting to study how HTLV-1 bZIP factor influences the expression and function of multiple co-inhibitory receptors to induce proliferation of infected T-cells."AuthorsKinosada H,Yasunaga JI,Shimura K,Miyazato P,Onishi C,Iyoda T,Inaba K,Matsuoka M

技术参数

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