CD253 (TRAIL) Monoclonal Antibody (N2B2), Functional Grade, eBioscience™/CD253 (TRAIL) 单克隆 (N2B2), 功能抗体, eBioscience™

货号：16-5951-38,16-5951-85,16-5951-025,16-5951-050

规格：5 mg,500 µg,25 mg,50 mg

价格：24089,4085,68175,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD253

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：N2B2

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1 µg/test（Flow）;Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The N2B2 monoclonal antibody reacts with mouse TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily. TRAIL is not detected on the surface of freshly isolated T, B, or NK cells, but can be induced preferentially on CD3^- NK1.1^+ NK cells after stimulation with IL-2 or IL-15. N2B2 inhibits IL-2- or IL-15-activated NK cell cytotoxicity against mouse fibrosarcoma L929 target cells.Applications Reported: N2B2 has been reported for use in flow cytometricanalysis, and blocking of TRAIL in functional assays.Applications Tested: The N2B2 antibody has been tested by flow cytometric analysis of mouse TRAIL transfected cells and can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Receptors for TRAIL include two death domain containing receptors, death receptor 4 (DR4) and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. TRAIL is a type II membrane protein and expressed in a variety of human tissues. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.仅用于科研。不用于诊断过程。

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参考文献：

流式1.Gene therapyInterleukin-15 liver gene transfer increases the number and function of IKDCs and NK cells."16-5951 was used in Flow cytometry/Cell sorting to show the novel functional link between circulating IL-15 and interferon (IFN)-producing killer dendritic cells (IKDCs)."AuthorsArina A,Murillo O,Dubrot J,Azpilikueta A,Gabari I,Perez-Gracia JL,Alfaro C,Berasain C,Prieto J,Ferrini S,Hervas-Stubbs S,Melero I2.Cancer researchMigratory and antigen presentation functions of IFN-producing killer dendritic cells."16-5951 was used in Flow cytometry/Cell sorting to study how BM-IKDCs are capable of cytotoxic killing of tumour targets, and also of potent antigen presentation after encountering antigen in the context of a viable target cell."AuthorsHimoudi N,Yan M,Bouma G,Morgenstern D,Wallace R,Seddon B,Buddle J,Eddaoudi A,Howe SJ,Cooper N,Anderson J3.Proceedings of the National Academy of Sciences of the United States of AmericaModulation of natural killer cell antitumor activity by the aryl hydrocarbon receptor."16-5951 was used in Flow cytometry/Cell sorting to explore the regulation of natural killer cells by the aryl hydrocarbon receptor (AhR)."AuthorsShin JH,Zhang L,Murillo-Sauca O,Kim J,Kohrt HE,Bui JD,Sunwoo JB中和4.Cell death and diseaseRIPK1 promotes death receptor-independent caspase-8-mediated apoptosis under unresolved ER stress conditions."Published figure using CD253 (TRAIL) monoclonal antibody (Product # 16-5951-85) in Neutralization"5.Journal of immunology (Baltimore, Md. : 1950)Immune unresponsiveness to secondary heterologous bacterial infection after sepsis induction is TRAIL dependent."16-5951 was used in Blocking experiments to implicate TRAIL-dependent immune suppression during sepsis, suggesting TRAIL neutralization may be a potential therapeutic target to restore cellular immunity."AuthorsGurung P,Rai D,Condotta SA,Babcock JC,Badovinac VP,Griffith TS6.Journal of virologyMonocytes treated with human immunodeficiency virus Tat kill uninfected CD4(+) cells by a tumor necrosis factor-related apoptosis-induced ligand-mediated mechanism."16-5951 was used in Neutralization experiments to show that production of TRAIL by Tat-stimulated monocytes provides a mechanism by which HIV infection can destroy uninfected bystander cells."AuthorsYang Y,Tikhonov I,Ruckwardt TJ,Djavani M,Zapata JC,Pauza CD,Salvato MS功能检测7.GutTumour necrosis factor related apoptosis inducing ligand (TRAIL) induces hepatic steatosis in viral hepatitis and after alcohol intake."16-5951 was used in Functional assays to investigate the role of tumour necrosis factor related apoptosis inducing ligand in viral hepatitis, and following alcohol consumption."AuthorsMundt B,Wirth T,Zender L,Waltemathe M,Trautwein C,Manns MP,Kühnel F,Kubicka S

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