CD335 (NKp46) Monoclonal Antibody (29A1.4), Functional Grade, eBioscience™/CD335 (NKp46) 单克隆 (29A1.4), 功能抗体, eBioscience™

货号：16-3351-85,16-3351-81,16-3351-38,16-3351-025,16-3351-050

规格：500 µg,50 µg,5 mg,25 mg,50 mg

价格：4324,1752,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD335

物种：人/小鼠/大鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：29A1.4

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1 µg/test（Flow）;Assay-Dependent（功能检测）

产品详细信息Description: The monoclonal antibody 29A1.4 recognizes mouse NKp46, also known as CD335. CD335, a member of the natural cytotoxicity receptor (NCR) family, is a glycoprotein with 2 Ig-like domains and a short cytoplasmic tail. Expression of CD335 is uniquely found on NK cells (including immature NK cells, defined as DX5- CD3-, and thereby allowing discrimination between NKT cells and NK cells (NKp46+, CD3-). Furthermore, unlike many of the NK markers which also stain NKT cells, staining with 29A1.4 is not strain specific. Staining has been shown on C57Bl/6, SJL, CBA/CA and BALB/C strains. NKp46 has been shown to play a role in NK cell-mediated lysis of several tumor cells and pathogen-infected cell lines.The 29A1.4 monoclonal antibody has been shown to activate NK cells in vitro. The 29A1.4 monoclonal antibody does not deplete NK cells in vivo.Applications Reported: This 29A1.4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 29A1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息NKp46 (CD335, NCR1, ) is a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. NKp46 consists of two Ig-like domains assembled to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).仅用于科研。不用于诊断过程。

数据

CD335 (NKp46) Antibody (16-3351-85) in FlowStaining of C57Bl/6 splenocytes with 0.5 µg of Rat IgG2a Isotype Control Purified (Product # 14-4321-82) (left) or 0.5 µg of Anti-Mouse CD335 (NKp46) Functional Grade Purified (right) followed by Anti-Rat IgG Biotin (Product # 13-4813-85), Streptavidin PE (Product # 12-4317-87), and finally Anti-Mouse CD49b (Integrin alpha 2) APC (Product # 17-5971-82).Total viable cells were used for analysis.

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参考文献：

流式1.Nature communicationsLoss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis."Published figure using CD335 (NKp46) monoclonal antibody (Product # 16-3351-81) in Flow Cytometry"2.Cellular and molecular immunologyThe breast tumor microenvironment alters the phenotype and function of natural killer cells."Published figure using CD335 (NKp46) monoclonal antibody (Product # 16-3351-81) in Flow Cytometry"3.Nature immunologyIL-22 bridges the lymphotoxin pathway with the maintenance of colonic lymphoid structures during infection with Citrobacter rodentium.AuthorsOta N,Wong K,Valdez PA,Zheng Y,Crellin NK,Diehl L,Ouyang W4.BloodLatent herpesvirus infection arms NK cells."16-3351 was used in Flow cytometry/Cell sorting to investigate the immune environment created by latent herpesvirus infection, and how this may provide a mechanism whereby host NK-cell function is enhanced in vivo.."AuthorsWhite DW,Keppel CR,Schneider SE,Reese TA,Coder J,Payton JE,Ley TJ,Virgin HW,Fehniger TAFN5.Journal of immunology (Baltimore, Md. : 1950)The natural cytotoxicity receptor NKp46 is dispensable for IL-22-mediated innate intestinal immune defense against Citrobacter rodentium."16-3351 was used in Functional assays to investigate the role of NKp46 cells in intestinal homeostasis and immune defence."AuthorsSatoh-Takayama N,Dumoutier L,Lesjean-Pottier S,Ribeiro VS,Mandelboim O,Renauld JC,Vosshenrich CA,Di Santo JP

技术参数

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