CD14 Monoclonal Antibody (61D3), Functional Grade, eBioscience™/CD14 单克隆 (61D3), 功能抗体, eBioscience™

货号：16-0149-82,16-0149-025 ,16-0149-050

规格：100 µg,25 mg,50 mg

价格：2872,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD14

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：61D3

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1 µg/test（Flow）;Assay-Dependent（功能检测）

产品详细信息Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.Applications Reported: The 61D3 antibody has been reported for use in flow cytometric analysis. 61D3 has also been reported for in vitro functional studies. (Fluorochrome-conjugated 61D3 is recommended for use in flow cytometry).Applications Tested: The 61D3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.仅用于科研。不用于诊断过程。

数据

CD14 Antibody (16-0149-82) in FlowStaining of normal human peripheral blood cells with Anti-Human CD14 FITC (left) or PE (right).Appropriate isotype controls were used (open histogram).Cells in the monocyte population were used for analysis.

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参考文献：

流式1.Frontiers in immunologyCharacterizing the Role of Monocytes in T Cell Cancer Immunotherapy Using a 3D Microfluidic Model."Published figure using CD14 monoclonal antibody (Product # 16-0149-82) in Flow Cytometry"2.Nature communicationsCellular metabolism constrains innate immune responses in early human ontogeny."Published figure using CD14 monoclonal antibody (Product # 16-0149-82) in Flow Cytometry"3.Oncology lettersButyrate upregulates the TLR4 expression and the phosphorylation of MAPKs and NK-κB in colon cancer cellin vitro."Published figure using CD14 monoclonal antibody (Product # 16-0149-82) in Flow Cytometry"IF4.Scientific reportsThrombomodulin regulates monocye differentiation via PKCδ and ERK1/2 pathway in vitro and in atherosclerotic artery."Published figure using CD14 monoclonal antibody (Product # 16-0149-82) in Flow Cytometry"5.Arthritis and rheumatology (Hoboken, N.J.)The role of transforming growth factor β signaling in fibroblast-like synoviocytes from patients with oligoarticular juvenile idiopathic arthritis: dysregulation of transforming growth factor β signaling, including overexpression of bone morphogenetic protein 4, may lead to a chondrocyte phenotype and may contribute to bony hypertrophy."Published figure using CD14 monoclonal antibody (Product # 16-0149-82) in Immunofluorescence"

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