CD11a (LFA-1alpha) Monoclonal Antibody (M17/4), Functional Grade, eBioscience™/CD11a (LFA-1alpha) 单克隆 (M17/4), 功能抗体, eBioscience™

货号：16-0111-82,16-0111-85,16-0111-38,16-0111-025,16-0111-050

规格：100 µg,500 µg,5 mg,25 mg,50 mg

价格：1752,3274,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD11a (LFA-1alpha)

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：M17/4

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）；Assay-Dependent（对照）；Assay-Dependent（FN）

产品详细信息Description: The M17/4 monoclonal antibody reacts with mouse CD11a, the 180 kDa integrin alpha L, also known as the lymphocyte function associated antigen-1 (LFA-1) alpha chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin beta 2), serves as an important adhesion molecule involved in lymphocyte and granulocyte function. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1. CD11a is expressed by all leukocytes.Applications Reported: The M17/4 antibody has been reported for use in flow cytometric analysis. M17/4 has also been reported in blocking of CD11a function.Applications Tested: The M17/4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. CD11a is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of CD11a to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. CD11a is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of CD11a function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti- CD11a antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. Two transcript variants encoding different isoforms of CD11a have been found. Diseases associated with CD11a dysfunction include benign cephalic histiocytosis and leukocyte adhesion deficiency.仅用于科研。不用于诊断过程。

数据

CD11a (LFA-1alpha) Antibody (16-0111-82) in FlowStaining of C57Bl/6 splenocytes with 0.25 µg of Rat IgG2a K Isotype Control Functional Grade Purified (Product # 16-4321-82) (open histogram) or 0.25 µg of Anti-Mouse CD11a Functional Grade Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85).Cells in the lymphocyte gate were used for analysis.

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参考文献：

流式1.PloS oneA Novel Model of Asymptomatic Plasmodium Parasitemia That Recapitulates Elements of the Human Immune Response to Chronic Infection."16-0111 was used in Flow cytometry/Cell sorting to investigate aspects of the human immune response to asymptomatic persistent Plasmodium infection using a novel animal model."AuthorsFontana MF,Baccarella A,Craft JF,Boyle MJ,McIntyre TI,Wood MD,Thorn KS,Anidi C,Bayat A,Chung MR,Hamburger R,Kim CY,Pearman E,Pham J,Tang JJ,Boon L,Kamya MR,Dorsey G,Feeney ME,Kim CC2.Shock (Augusta, Ga.)Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression."Published figure using CD11a (LFA-1alpha) monoclonal antibody (Product # 16-0111-82) in Flow Cytometry"3.Infection and immunityCD8+ T cell exhaustion, suppressed gamma interferon production, and delayed memory response induced by chronic Brucella melitensis infection."16-0111 was used in Flow cytometry/Cell sorting to assess the populations of immune cells which respond to chronic infection of Brucella melitensis."AuthorsDurward-Diioia M,Harms J,Khan M,Hall C,Smith JA,Splitter GA中和4.Nature communicationsTreg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration."16-0111 was used in Blocking experiments to studying Treg cell engagement with the lymphotoxin beta receptor in transendothelial migration."AuthorsBrinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JSIF5.PloS oneCalpain 4 is not necessary for LFA-1-mediated function in CD4+ T cells."16-0111 was used in Immunofluorescence to study calpain regulation of LFA-1 function."AuthorsWernimont SA,Simonson WT,Greer PA,Seroogy CM,Huttenlocher A

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