CD3e Monoclonal Antibody (145-2C11), Biotin, Functional Grade, eBioscience™/CD3e 单克隆 (145-2C11), Biotin, 功能抗体, eBioscience™

货号： 36-0031-85

规格：500 µg

价格：3669

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	IgG
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）；Assay-Dependent（IP）；Assay-Dependent（FN）

产品详细信息Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.Applications Reported: This 145-2C11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. It has also been reported for use in cell activation and cell depletion. Please visit the best Protocols webpage to view a protocol for in vitro T-cell activation.Applications Tested: This 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.05 ng/ug cytokine as determined by the LAL assay.Storage and handling: Use in a sterile environment.靶标信息The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components havelong cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.仅用于科研。不用于诊断过程。

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参考文献：

流式1.Frontiers in immunologyTpl2 Protects Against Fulminant Hepatitis Through Mobilization of Myeloid-Derived Suppressor Cells."Published figure using CD3e monoclonal antibody (Product # 36-0031-85) in Flow Cytometry"2.Journal of cellular and molecular medicineEnhanced alleviation of aGVHD by TGF-β1-modified mesenchymal stem cells in mice through shifting MΦ into M2 phenotype and promoting the differentiation of Treg cells."Published figure using CD3e monoclonal antibody (Product # 36-0031-85) in Flow Cytometry"WB3.Nature communicationsRabphilin 3A retains NMDA receptors at synaptic sites through interaction with GluN2A/PSD-95 complex."Published figure using CD3e monoclonal antibody (Product # 36-0031-85) in Immunofluorescence"IHC4.Nature communicationsAge-specific biological and molecular profiling distinguishes paediatric from adult acute myeloid leukaemias."Published figure using CD3e monoclonal antibody (Product # 36-0031-85) in Flow Cytometry"5.Nature communicationsObesity exacerbates colitis-associated cancer via IL-6-regulated macrophage polarisation and CCL-20/CCR-6-mediated lymphocyte recruitment."Published figure using CD3e monoclonal antibody (Product # 36-0031-85) in Immunocytochemistry"

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