CD28 Monoclonal Antibody (CD28.6), Functional Grade, eBioscience™/CD28 单克隆 (CD28.6), 功能抗体, eBioscience™

货号：16-0288-81,16-0288-85,16-0288-38,16-0288-025,16-0288-050

规格：50 µg,500 µg,5 mg,25 mg,50 mg

价格：1283,3767,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD28

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：CD28.6

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1 µg/test（Flow）;Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The CD28.6 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells. The CD28.6 antibody has been reported for use in immunoblotting (WB) and neutralization studies and costimulation in the production of IL-2.Applications Reported: The CD28.6 antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB). The recommended titer for WB is (~1 µ/mL). CD28.6 has also been reported in in vitro functional assays, both blocking and co-stimulatory activities have been reported for this clone (please see references below).Applications Tested: TheCD28.6 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing Tcells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.仅用于科研。不用于诊断过程。

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参考文献：

流式1.BloodDendritic cells accumulate in the bone marrow of myeloma patients where they protect tumor plasma cells from CD8+ T-cell killing."16-0288 was used in Flow cytometry/Cell sorting to suggest that DCs activate CD81 T-cells against tumor plasma cells, but nullify this activation by protecting tumor plasma cells."AuthorsLeone P,Berardi S,Frassanito MA,Ria R,De Re V,Cicco S,Battaglia S,Ditonno P,Dammacco F,Vacca A,Racanelli V2.Arthritis research and therapySynovial IL-9 facilitates neutrophil survival, function and differentiation of Th17 cells in rheumatoid arthritis."16-0288 was used in Flow cytometry/Cell sorting to show synovial IL-9 may contribute to rheumatoid arthritis by prolonging neutrophil survival, increasing expression of neutrphil-matrix metalloprotienase-9 and facilitating Th17 cell differentiation."AuthorsChowdhury K,Kumar U,Das S,Chaudhuri J,Kumar P,Kanjilal M,Ghosh P,Sircar G,Basyal RK,Kanga U,Bandyopadhaya S,Mitra DK中和3.The Journal of allergy and clinical immunologyIL-4 confers resistance to IL-27-mediated suppression on CD4+ T cells by impairing signal transducer and activator of transcription 1 signaling."16-0288 was used in Neutralization experiments to explore the mechanisms by which CD4(+) T cells from asthmatic patients resist IL-27-mediated inhibition."AuthorsChen Z,Wang S,Erekosima N,Li Y,Hong J,Qi X,Merkel P,Nagabhushanam V,Choo E,Katial R,Alam R,Trikha A,Chu HW,Zhuang Y,Jin M,Bai C,Huang H功能检测4.PloS oneLarge-Scale Purification of r28M: A Bispecific scFv Antibody Targeting Human Melanoma Produced in Transgenic Cattle."16-0288 was used in an ELISA assay to study how the bispecific scFv antibody r28M produced in transgenic cattle targets human melanoma."AuthorsSpiesberger K,Paulfranz F,Egger A,Reiser J,Vogl C,Rudolf-Scholik J,Mayrhofer C,Grosse-Hovest L,Brem G5.BloodFatal autoimmunity in mice reconstituted with human hematopoietic stem cells encoding defective FOXP3."16-0288 was used in Functional assays to develop mouse stains that express human leukocyte antigen DR1 rather than murine MHC class II, displaying fatal autoimmunity following reconstituted with human hematopoietic stem cells encoding defective FOXP3."AuthorsGoettel JA,Biswas S,Lexmond WS,Yeste A,Passerini L,Patel B,Yang S,Sun J,Ouahed J,Shouval DS,McCann KJ,Horwitz BH,Mathis D,Milford EL,Notarangelo LD,Roncarolo MG,Fiebiger E,Marasco WA,Bacchetta R,Quintana FJ,Pai SY,Klein C,Muise AM,Snapper SB

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