| Achieve fast, versatile, and reliable fluorophore-, biotin-, or enzyme-labeled IgG primary antibodies with the Zenon rabbit IgGlabelingkits. These kits utilize Alexa Fluorfluorophores, biotin,Pacific Blue,or enzymes such asR-phycoerythrinandallophycocyanin, which are attached to monovalent, affinity purified Fab fragments.The Fab fragments, in turn, are directed against and bind with the Fc portion of IgG primary antibodies.Only a small amount of starting materialis required, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions.Because theZenonlabelingmethodis based onimmunoselectivity,itdoes not requiretheremoval of exogenous proteins(such as serum)or amine-containing buffers from the target antibody, simplifying the process.Zenon labeling technology greatly simplifies the use of multiple mouse-derived antibodies in the same staining protocol. Zenontricolorlabelingkits contain sufficient materials for 10 labeling reactions of each of three different fluorescent colors.Cost savingsZenon antibody labeling kits offer a cost-conscious and reproducible method of tagging as little as 0.4 µg in 2 µL of primary antibody, with minimal waste of expensive or difficult-to-obtain antibodies, or excessive washing steps that pose the risk of product loss.SensitivityLabel your primary antibodies without compromising their antigen binding affinity: Zenon dye- and enzyme-labeled Fab fragments, which are targeted to the Fc tail, are affinity purified during their preparation to ensure high affinity and selectivity for the Fc portion of the corresponding primary antibody. The procedure for chemical labeling of the Zenon Fab fragments protects their Fc-binding site, resulting in more active labeling reagents.SpeedNo purification procedure is required prior to using Zenon Fab fragments in your laboratory applications. Formation of the Fab-antibody complex occurs in fewer than five minutes, followed by a five-minute blocking step. During this time, almost all the primary antibody in the mixture is labeled with the labeled Fab fragments.SimplicityThe Fab-antibody complexes display fluorescence or enzymatic activity that is similar in intensity to that of directly labeled primary antibodies. Varying the extent of the antibody labeling is as simple as changing the amount of added Zenon labeling reagent during the reaction. Once the labeling complexes are formed, they can used immediately, without need for antibody purification.ReliabilityThe Zenon Fab-antibody complex is stable and allows subsequent or simultaneous labeling of different target cells and tissues with different complexes. After staining, an aldehyde-based fixing step may be used to prevent the transfer of different labels between different primary antibodies, preserving the initial staining pattern.CustomizationWe offer custom antibody conjugation services that are efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified. |
| • Labeled antibodies are typically ready to use in 10 minutes• Requires only 1–20 µg primary antibody• Simple—no purification required• Flexible — choose from different fluorophores, biotin, HRP, alkaline phosphatase• Multiplex with other mouse monoclonal antibodies simultaneously• Can be used in a variety of applications including ICC, IHC, flow cytometry, and cell imaging. |
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