Fluoresceinyl Glycine Amide (5-(Aminoacetamido)Fluorescein)/荧光素甘氨酸酰胺(5-(氨基乙酰氨基)荧光素)
货号:A1363
规格:10 mg
价格:4220
产品类型:蛋白标记
品牌:Thermo Fisher
The primary aliphatic amine of5-(aminoacetamido)fluorescein (fluoresceinyl glycine amide)can be reversibly coupled to aldehydes and ketones to form a Schiff base - which can be reduced to a stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3) to form new probes. Carboxylic acids of proteins and other water-soluble biopolymers can be coupled to this molecule in aqueous solution using water-soluble carbodiimides such as EDAC (E2247). The glycine may be the better amine probe for direct carbodiimide-mediated coupling, since it is likely to remain unprotonated at a lower pH than other aliphatic amines.
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