Cardiac Troponin I (TNNI3) Human ELISA Kit/人心肌肌钙蛋白I（TNNI3）ELISA试剂盒
货号：EHTNNI3,EHTNNI3X5,EHTNNI3X10
规格：96 tests,5X96 tests,10X96 tests
价格：7311,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 100 pg/mL |
| 检测范围 | 125-8000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | Cardiac Troponin I |
| Protein aliases | Cardiac troponin I, cardiomyopathy, dilated 2A (autosomal recessive), troponin I type 3 (cardiac), Troponin I, cardiac muscle |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | CMD1FF, CMD2A, CMH7, cTnI, RCM1, TNNC1, TNNI3 |
| Gene ID | (Human) 7137 |
| Gene symbol | TNNI3 |
| UniProt ID | (Human) P19429 |
| The Human Troponin I (TNNI3) ELISA quantitates Hu TNNI3 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TNNI3.人肌钙蛋白I（TNNI3）ELISA定量了人血清，血浆或细胞培养基中的人TNNI3。 该检测试剂盒将专门识别天然和重组人TNNI3。Principle of the method 方法原理The Human TNNI3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人TNNI3固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Cardiac Troponin I (Cardiac-specific troponin I, TnI, Troponin I) is an integral inhibitory protein in cardiac muscle that exists as part of a complex with troponin C (TnC) and troponin T (TnT). The TnT subunit of troponin binds to tropomyosin-t form, a troponin-tropomyosin complex, anchored in place by the binding of TnI to actin, within muscle thin filaments. Structural change resulting from the binding of calcium to specific sites on the regulatory TnC subunit, releases the inhibitory region of TnI from actin, anabling the attachment of the molecular motor protein myosin, allowing for muscle contraction and hence movement. The measurements of blood TnI and TnT levels is an important diagnostics indicator of heart muscle damage, and can be used to differentiate between angina and myocardial infarction in patients with chest pains. Cardiac Troponin I has also been shown to inhibit angiogenesis in vivo and in vitro. Mutations in the Cardiac Troponin I gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).心肌肌钙蛋白I（心肌特异性肌钙蛋白I，TnI，肌钙蛋白I）是心肌中不可或缺的抑制蛋白，与肌钙蛋白C（TnC）和肌钙蛋白T（TnT）形成复合物。肌钙蛋白的TnT亚基结合于肌细丝内的肌钙蛋白-t形式，一种肌钙蛋白-肌球蛋白复合物，通过TnI与肌动蛋白的结合而锚定在适当的位置。由于钙与调节性TnC亚基上特定位点的结合而导致的结构变化，从肌动蛋白释放了TnI的抑制区，使分子运动蛋白肌球蛋白的附着得以实现，从而使肌肉收缩并因此运动。血液TnI和TnT水平的测量是心肌损伤的重要诊断指标，可用于区分胸痛患者的心绞痛和心肌梗塞。心肌肌钙蛋白I也已显示在体内和体外抑制血管生成。心肌肌钙蛋白I基因的突变会导致7型家族性肥厚性心肌病（CMH7）和家族性限制性心肌病（RCM）。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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