CD4 Monoclonal Antibody (GK1.5), eFluor 450
货号：48-0041-80,48-0041-82
规格：25μg,100μg
价格：1038,2271
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2b, kappa
荧光染料：eFluor 450
| 抗体类型 | 单抗 | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor 450 |
| 浓度： | 0.2 mg/mL | 用法： | 0.25 µg/test (Flow) |
| 产品详细信息Description: The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.Applications Reported: This GK1.5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This GK1.5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017]. |
| 数据 |
| CD4 Antibody (48-0041-80) in FlowStaining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2b K Isotype Control eFluor® 450 (Product # 48-4031-82) (left) or 0.125 µg of Anti-Mouse CD4 eFluor® 450 (right). Total viable cells were used for analysis. |
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| 参考文献： |
| 1.Scientific reportsHeavy Chain-Hyaluronan/Pentraxin 3 from Amniotic Membrane Suppresses Inflammation and Scarring in Murine Lacrimal Gland and Conjunctiva of Chronic Graft-versus-Host Disease."Published figure using CD4 monoclonal antibody (Product # 48-0041-82) in Immunofluorescence"2.Scientific reportsEpithelial EP4 plays an essential role in maintaining homeostasis in colon."Published figure using CD4 monoclonal antibody (Product # 48-0041-82) in Immunohistochemistry"3.Molecular medicine (Cambridge, Mass.)Rapamycin ameliorates dystrophic phenotype in mdx mouse skeletal muscle."Published figure using CD4 monoclonal antibody (Product # 48-0041-82) in Immunohistochemistry"4.Digestive diseases and sciencesSomatostatin inhibits the production of interferon-γ by intestinal epithelial cells during intestinal ischemia-reperfusion in macaques."Published figure using CD4 monoclonal antibody (Product # 48-0041-82) in Immunohistochemistry"5.AutophagyB cell autophagy mediates TLR7-dependent autoimmunity and inflammation."Published figure using CD4 monoclonal antibody (Product # 48-0041-82) in Immunohistochemistry" |
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