| 产品详细信息Description: The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naive mice, GITR is expressed predominantly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes. Stimulation of GITR with DTA-1 antibody abrogates Treg cell-mediated suppression. Although it does not deplete CD357 expressing cells in vivo, it has been shown to induce tumorogenesis. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease.Applications Reported: The DTA-1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.004 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. It is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells. Knockout studies in mice also suggest the roleof this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| 1.Journal of immunology (Baltimore, Md. : 1950)Pegylated bisacycloxypropylcysteine, a diacylated lipopeptide ligand of TLR6, plays a host-protective role against experimental Leishmania major infection."12-5874 was used in Flow cytometry/Cell sorting to investigate the functional differences in different TLR heterodimers."AuthorsPandey SP,Chandel HS,Srivastava S,Selvaraj S,Jha MK,Shukla D,Ebensen T,Guzman CA,Saha B2.Journal of immunology (Baltimore, Md. : 1950)CD40 expression levels modulate regulatory T cells in Leishmania donovani infection."12-5874 was used in Flow cytometry/Cell sorting to investigate the role of dendritic cell-expressed CD40 in the generation of Tregs."AuthorsMartin S,Agarwal R,Murugaiyan G,Saha B3.European journal of microbiology and immunologyImpact of enzymatic tissue disintegration on the level of surface molecule expression and immune cell function."12-5874 was used in Flow cytometry/Cell sorting to indicate that enzymatic tissue disintegration can have profound effects on the expression of a variety of cell-surface molecules."AuthorsAutengruber A,Gereke M,Hansen G,Hennig C,Bruder D4.PloS oneGlutamic acid decarboxylase-derived epitopes with specific domains expand CD4(+)CD25(+) regulatory T cells."12-5874 was used in Flow cytometry/Cell sorting to investigate how structural analysis of epitopes may aid predictions of protective epitopes to expand functional Tregs."AuthorsChen G,Han G,Feng J,Wang J,Wang R,Xu R,Shen B,Qian J,Li Y5.Arthritis and rheumatology (Hoboken, N.J.)Interleukin-21 promotes germinal center reaction by skewing the follicular regulatory T cell to follicular helper T cell balance in autoimmune BXD2 mice."12-5874 was used in Flow cytometry/Cell sorting to indicate that high levels of IL-21 selectively enhance Tfh cell differentiation but inhibit Tfr cell commitment and the suppressive function of Tfr cells on Tfh cells and B cells."AuthorsDing Y,Li J,Yang P,Luo B,Wu Q,Zajac AJ,Wildner O,Hsu HC,Mountz JD |
QQ:1749072012