Armenian Hamster IgG Isotype Control (eBio299Arm), eBioscience™
货号：14-4888-81,14-4888-85
规格：50 µg,500 µg
价格：582,1514
产品类型：同型对照
品牌：eBioscience
物种：其它
宿主：Armenian hamster仓鼠
抗体亚型：IgG
克隆号：eBio299Arm
荧光染料：其它
| 抗体类型 | 仓鼠同型对照 | 同型对照： | IgG |
| 浓度： | 0.2 mg/mL | 用法： | Assay-Dependent（IHC）;Assay-Dependent(ICC)；Assay-Dependent（IF）；Assay-Dependent（IP）;Assay-Dependent(IA);Assay-Dependent(Flow)；Assay-Dependent(Ctrl) |
| 产品详细信息Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: This eBio299Arm antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry.Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood. Use isotype control at the same concentration as experimental antibody.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization. |
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| 参考文献： |
| 1.Cancer cellApo2L/TRAIL and the death receptor 5 agonist antibody AMG 655 cooperate to promote receptor clustering and antitumor activity."14-4888 was used in Immunohistochemistry to provide insight into the limited efficacy observed in previous clinical trials and suggest testable hypotheses to reconsider death receptor agonism as a therapeutic strategy."2.PloS oneAn evolutionary-conserved function of mammalian notch family members as cell adhesion molecules."14-4888 was used in Flow cytometry/Cell sorting to demonstrate that the function of cell adhesion is conserved in mammalian and Drosophila Notch family members."3.OncoimmunologyPoly(I:C) and CpG-ODN combined aerosolization to treat lung metastases and counter the immunosuppressive microenvironment."14-4888 was used in Flow cytometry/Cell sorting to evaluated aerosol delivery CpG-ODN combined with Poly(I:C) in the treatment of B16 melanoma lung metastases in C57BL/6 mice."4.Biochimica et biophysica actaAltered leukotriene B4 metabolism in CYP4F18-deficient mice does not impact inflammation following renal ischemia."14-4888 was used in Flow cytometry/Cell sorting to conclude that CYP4F18 is necessary for omega oxidation of leukotriene B4 in neutrophils, and is not compensated by other CYP enzymes." |
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