| 产品详细信息Description: The OX-86 monoclonal antibody reacts with mouse CD134, also known as OX40. A member of the TNF receptor superfamily, CD134 is a 50 kDa type I membrane glycoprotein expressed by activated mouse T lymphocytes. Rat CD134 was initially identified as an activation marker only on activated CD4+ T cells. In contrast, mouse CD134 is expressed by both activated CD4+ and CD8+ T cells. The interaction of CD134 with CD252 (OX40 ligand) has been implicated in T cell-dependent humoral responses,regulation of primary T cell expansion, survival of T cells, size of the memory T cell pool, and regulation of tolerance in the CD4+ T cell compartment.Applications Reported: This OX-86 antibody has been reported for use in flow cytometric analysis.Applications Tested: This OX-86 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Tumor necrosis factor receptor superfamily, member4, also known as ACT35 or CD134 is a cell surface glycoprotein that was discovered through the production of a monoclonal antibody raised against the HUT-102 cell line. It belongs to the tumor necrosis factor receptor superfamily. CD134 was mapped to 1p36 by fluorescence in situ hybridization. CD134 is the primary receptor for feline immunodeficiency virus. CD134 expression can promote viral binding and renders cells permissive for viral entry, productive infection, and syncytium formation. Stimulating the receptor can improve the response to a powerful virus vector and may be useful in vaccine development. |
| 1.Cancer researchAdenovirus vector-mediated in vivo gene transfer of OX40 ligand to tumor cells enhances antitumor immunity of tumor-bearing hosts.AuthorsAndarini S,Kikuchi T,Nukiwa M,Pradono P,Suzuki T,Ohkouchi S,Inoue A,Maemondo M,Ishii N,Saijo Y,Sugamura K,Nukiwa T2.Frontiers in immunologyLimited Foxp3+Regulatory T Cells Response During AcuteTrypanosoma cruziInfection Is Required to Allow the Emergence of Robust Parasite-Specific CD8+T Cell Immunity."17-1341 was used in Flow cytometry/Cell sorting to study the role of regulatory T cells during Trypanosoma cruzi infection."AuthorsAraujo Furlan CL,Tosello Boari J,Rodriguez C,Canale FP,Fiocca Vernengo F,Boccardo S,Beccaria CG,Adoue V,Joffre O,Gruppi A,Montes CL,Acosta Rodriguez EV3.Cell reportsPulsatile MEK Inhibition Improves Anti-tumor Immunity and T Cell Function in Murine Kras Mutant Lung Cancer."17-1341 was used in Flow cytometry/Cell sorting to set the foundation and show the importance of a combinatorial therapeutic strategy using pulsatile targeted therapy together with immunotherapy to optimally enhance tumor delay and promote long-term anti-tumor immunity."AuthorsChoi H,Deng J,Li S,Silk T,Dong L,Brea EJ,Houghton S,Redmond D,Zhong H,Boiarsky J,Akbay EA,Smith PD,Merghoub T,Wong KK,Wolchok JD4.Journal of immunology (Baltimore, Md. : 1950)The Ox40/Ox40 Ligand Pathway Promotes Pathogenic Th Cell Responses, Plasmablast Accumulation, and Lupus Nephritis in NZB/W F1 Mice."Published figure using CD134 (OX40) monoclonal antibody (Product # 17-1341-82) in Flow Cytometry"5.PloS oneRoscovitine suppresses CD4+ T cells and T cell-mediated experimental uveitis."17-1341 was used in Flow cytometry/Cell sorting to test the immunosuppressive effect of roscovitine, a potent CdK2 inhibitor, on CD4+ T cell activation, proliferation, and function."AuthorsZhang Z,Liu Q,Leskov KS,Wu X,Duan J,Zhang GL,Hall M,Rosenbaum JT |
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