CD8a Monoclonal Antibody (OKT8 (OKT-8)), APC, eBioscience™
货号：17-0086-41,17-0086-42
规格：25tests,100tests
价格：1244,2683
产品类型：流式抗体
品牌：Thermo Fisher
抗原：CD8a
物种：人
宿主：小鼠
抗体亚型：IgG2a, kappa
克隆号：OKT8 (OKT-8)
荧光染料：APC
| 抗体类型: | 流式抗体 | 同型对照： | Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience |
| 浓度： | 5 µL/Test | 用法： | 5 µL (0.06 µg)/test |
| Description:The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Preliminary testing indicates that OKT8 and two other mouse anti-human CD8 antibodies (clone RPA-T8, Cat. No.14-0088 and clone HIT8a, Cat. No.14-0089) do not compete with each other for binding to human peripheral blood leukocytes by flow cytometric analysis, suggesting that they do not bind to similar epitopes or block each other by steric hindrance.Applications Reported: This OKT8 (OKT-8) antibody has been reported for use in flow cytometric analysis.Applications Tested: This OKT8 (OKT-8) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization. |
| 数据 |
CD8a Antibody (17-0086-41) in FlowNormal human peripheral blood cells were stained with CD19 Monoclonal Antibody, PE (Product # 12-0199-42) and Mouse IgG2a kappa Isotype Control, APC (Product # 17-4724-81) (left) or CD8a Monoclonal Antibody, APC (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1. PloS oneSerum response factor regulates immediate early host gene expression in Toxoplasma gondii-infected host cells."17-0086 was used in Flow cytometry/Cell sorting to investigate the effect of Toxoplasma infection on Serum Response Factor activation."AuthorsWiley M,Teygong C,Phelps E,Radke J,Blader IJ2.Experimental animalsNOD-Rag2null IL-2Rγnull mice: an alternative to NOG mice for generation of humanized mice."17-0086 was used in Flow cytometry/Cell sorting to evaluate the usefulness of NR2G mice as humanised mice by comparing human cell engraftment and differentiation among NOG, NR2G, and NOD-scid mice after HSC transplantation."AuthorsKatano I,Ito R,Kamisako T,Eto T,Ogura T,Kawai K,Suemizu H,Takahashi T,Kawakami Y,Ito M3.PloS oneMHC multimer-guided and cell culture-independent isolation of functional T cell receptors from single cells facilitates TCR identification for immunotherapy."Published figure using CD8a monoclonal antibody (Product # 17-0086-42) in Flow Cytometry" |
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