CD8a Monoclonal Antibody (RPA-T8), PE-Cyanine7, eBioscience™

货号：25-0088-41,25-0088-42

规格：25tests,100tests

价格：1518,3035

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD8a

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：RPA-T8

荧光染料：PE-Cy7

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test (Flow）

产品详细信息Description:The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.Applications Reported: This RPA-T8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RPA-T8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD8a Antibody (25-0088-41) in FlowStaining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human CD8a PE-Cyanine7 (filled histogram). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Journal of leukocyte biologySepsis-induced human lymphocyte apoptosis and cytokine production in "humanized" mice."Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry"2.Nature communicationsRSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection."Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry"3.Stem cell reportsLack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors."Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry"

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