CD2 Monoclonal Antibody (RPA-2.10), eBioscience™

货号：14-0029-82

规格：100 µg

价格：1156

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD2

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号： RPA-2.10

荧光染料：其它

抗体类型:	流式抗体	同型对照：	IgG1, kappa
浓度：	0.5 mg/mL	用法：	0.5 µg/test (Flow）

产品详细信息Description:The RPA-2.10 monoclonal antibody reacts with human CD2, a 50 kDa cell surface receptor expressed by a majority of thymocytes, all mature T cells and subset of NK cells. CD2 is a ligand for CD58 in the human and is involved in adhesion and activation of T cells. RPA-2.10 blocks mixed lymphocyte reaction.RPA-2.10 crossreacts to non-human primates and pigs.Applications Reported: The RPA-2.10 antibody has been reported for use in flow cytometric analysis, immunoblotting(WB), and immunohistochemical staining of frozen tissue sections. RPA-2.10 has also been reported for use in in vitro blocking of human CD2 (LFA-2). (Please use Functional Grade purified RPA-2.10, cat. 16-0029, in functional assays.).Applications Tested: This RPA-2.10 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD2 (LFA-2) is a monomeric surface antigen (MW range 45-58 kDa) of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells. CD2 is one of the earliest T-cell markers, being present on more than 95% of thymocytes and it is also found on some natural killer cells, but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. The interaction between CD2 and CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection. Diseases associated with CD2 dysfunction include penis squamous cell carcinoma and immune deficiency due to the absence of the thymus.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD2 Antibody (14-0029-82) in FlowStaining of normal human peripheral blood cells with 0.25 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Human CD2 Purified (filled histogram) followed by Anti-Mouse IgG PE (Product # 12-4012). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.The Journal of experimental medicineThe transcription factor E4bp4/Nfil3 controls commitment to the NK lineage and directly regulates Eomes and Id2 expression."14-0029 was used in Flow cytometry/Cell sorting to propose that E4bp4 is required for commitment to the NK lineage and can promote NK development by directly regulating the expression of the downstream transcription factors Eomes and Id2."2.Journal of immunology (Baltimore, Md. : 1950)Ebf1 and c-Myb repress rag transcription downstream of Stat5 during early B cell development."14-0029 was used in Flow cytometry/Cell sorting to identify a novel pathway responsible for the transcriptional repression of Rag in developing B cells using an in vitro model system."3.Epigenetics and chromatinGeneration of bivalent chromatin domains during cell fate decisions."14-0029 was used in Flow cytometry/Cell sorting to investigate the patterns and levels of histone modifications at bivalent genes, showing the generation of bivalent chromatin domains during cell fate decisions."4.The journal of histochemistry and cytochemistry : official journal of the Histochemistry SocietyA monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.

技术参数

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