CD4 Monoclonal Antibody (RPA-T4), Alexa Fluor 700
货号：56-0049-41,56-0049-42
规格：25 Tests,100 Tests
价格：1019,2482
产品类型：流式抗体
品牌：Thermo Fisher
物种：人
宿主：小鼠
抗体亚型：IgG1, kappa
荧光染料：Alexa Fluor 700
| 抗体类型 | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor 700 |
| 浓度： | 5 µL/Test | 用法： | 5 μL (0.25 μg)/test (Flow) |
| 产品详细信息Description: The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.Applications Reported: This RPA-T4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RPA-T4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017]. |
| 数据 |
| CD4 Antibody (56-0049-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 PE (Product # 12-0199-80) and Mouse IgG1 K Isotype Control Alexa Fluor® 700 (Product # 56-4714-80) (left) or Anti-Human CD4 Alexa Fluor® 700 (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Proceedings of the National Academy of Sciences of the United States of AmericaRemarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions."Published figure using CD4 monoclonal antibody (Product # 56-0049-42) in Flow Cytometry"2.Cancer immunology, immunotherapy : CIIIdentification of two HLA-A*0201 immunogenic epitopes of lactate dehydrogenase C (LDHC): potential novel targets for cancer immunotherapy."Published figure using CD4 monoclonal antibody (Product # 56-0049-42) in Flow Cytometry"3.Frontiers in immunologyImpact of Enhancer of Zeste Homolog 2 on T Helper Cell-Mediated Allergic Rhinitis."Published figure using CD4 monoclonal antibody (Product # 56-0049-42) in Flow Cytometry"4.Molecular medicine reportsMicroRNA‑155 inhibits the proliferation of CD8+ Tcells via upregulating regulatory T cells in vitiligo."Published figure using CD4 monoclonal antibody (Product # 56-0049-42) in Flow Cytometry"5.CellRAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses."Published figure using CD4 monoclonal antibody (Product # 56-0049-42) in Flow Cytometry" |
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